Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells
Abstract
:1. Introduction
2. Results
2.1. MSTN Gene Inactivation Delayed the Senescence of Long-Term Cultured bSMCs
2.2. Inactivation of the MSTN Alleviated Senescence Caused by the Long-Term Culture of bSMCs
2.3. Inactivation of the MSTN Downregulated the SASP in Skeletal Muscle Senescence Cells
2.4. Inactivation of the MSTN Downregulated the SASP through the Upregulation of TREX1
2.5. Overexpression of TREX1 Can Rejuvenate Aged bSMCs
2.6. Aging Prematurely Due to TREX1 Absence in bSMCs
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Isolation
4.2. Telomere Length Assay
4.3. SA-β-Gal Staining
4.4. Immunofluorescent Staining
4.5. The use of Fluorescence-Activated Cell Sorting for Cell Cycle Assay (FACS)
4.6. Real-Time PCR
4.7. Western Blot
4.8. RNA-Seq Analysis
4.9. Chromatin Immunoprecipitation Assay
4.10. Luciferase Reporter
4.11. Transfection with TREX1 shRNA and Overexpression Vector
4.12. Statistics Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yang, M.; Gao, L.; Gao, Y.; Hao, Z.; Zhou, X.; Su, G.; Bai, C.; Wei, Z.; Liu, X.; Yang, L.; et al. Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells. Int. J. Mol. Sci. 2024, 25, 5277. https://doi.org/10.3390/ijms25105277
Yang M, Gao L, Gao Y, Hao Z, Zhou X, Su G, Bai C, Wei Z, Liu X, Yang L, et al. Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells. International Journal of Molecular Sciences. 2024; 25(10):5277. https://doi.org/10.3390/ijms25105277
Chicago/Turabian StyleYang, Miaomiao, Li Gao, Yajie Gao, Zhenting Hao, Xinyu Zhou, Guanghua Su, Chunling Bai, Zhuying Wei, Xuefei Liu, Lei Yang, and et al. 2024. "Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells" International Journal of Molecular Sciences 25, no. 10: 5277. https://doi.org/10.3390/ijms25105277