O-GlcNAc Modification Is a Promising Therapeutic Target for Diabetic Retinopathy
Abstract
:1. Introduction
2. Results
2.1. OGT and OGA Expression Changes Related to Diabetic Retinopathy
2.2. Effects of AMPK Activation on O-GlcNAc Modification Changes That Lead to Photoreceptor Cell Damage in Diabetic Retinopathy
2.3. Photoreceptor Cell Damage In Vitro Is Related to O-GlcNAc Modification Changes after High Glucose Treatment
2.4. AMPK Activation Affects 661 W Cell Apoptosis via O-GlcNAc Modification Changes after High Glucose Treatment
2.5. Effects of Attenuating 661 W Cell Apoptosis on Neovascularization during High Glucose Treatment In Vitro
2.6. AMPK Activation Attenuates the Expression of GFAT and TXNIP-O-GlcNAc Modification Changes in 661 W Cell Apoptosis after High Glucose Treatment In Vitro
3. Discussion
4. Materials and Methods
4.1. Bioinformatics Analysis
4.2. Animal
4.3. Cell Culture and Reagents
4.4. Hematoxylin–Eosin Staining and Morphology Analysis
4.5. Electroretinography (ERG)
4.6. Cell Viability Assay
4.7. Immunofluorescence Staining
4.8. Western Blot
4.9. TUNEL Staining
4.10. Tube Formation Assay
4.11. CO-IP Assay
4.12. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dong, W.; Imdad, L.; Xu, S.; Wang, Y.; Liu, C.; Song, S.; Li, Z.; Kong, Y.; Kong, L.; Ren, X. O-GlcNAc Modification Is a Promising Therapeutic Target for Diabetic Retinopathy. Int. J. Mol. Sci. 2024, 25, 6286. https://doi.org/10.3390/ijms25116286
Dong W, Imdad L, Xu S, Wang Y, Liu C, Song S, Li Z, Kong Y, Kong L, Ren X. O-GlcNAc Modification Is a Promising Therapeutic Target for Diabetic Retinopathy. International Journal of Molecular Sciences. 2024; 25(11):6286. https://doi.org/10.3390/ijms25116286
Chicago/Turabian StyleDong, Wenkang, Laraib Imdad, Shengnan Xu, Yinli Wang, Chengzhi Liu, Shiyu Song, Zechuan Li, Ying Kong, Li Kong, and Xiang Ren. 2024. "O-GlcNAc Modification Is a Promising Therapeutic Target for Diabetic Retinopathy" International Journal of Molecular Sciences 25, no. 11: 6286. https://doi.org/10.3390/ijms25116286