Genome-Wide Identification and Expression Analysis of the GRAS Gene Family and Their Responses to Heat Stress in Cymbidium goeringii
Abstract
:1. Introduction
2. Results
2.1. Identification and Physicochemical Properties of the GRAS Gene Family in C. goeringii
2.2. Phylogenetic Analysis of GRAS Genes
2.3. Gene Structure and Motif Analysis of GRAS Genes
2.4. Collinearity and Chromosomal Localization of CgGRAS Genes
2.5. Cis-Acting Regulatory Elements and Gene Ontology Analysis of GRAS Genes
2.6. The qRT-PCR Analysis of CgGRASs
3. Discussion
4. Materials and Methods
4.1. Data Sources
4.2. Treatment of Plant Materials
4.3. Identification and Physicochemical Properties of the GRAS Proteins
4.4. Phylogenetic Analysis of GRAS Genes
4.5. Gene Structure and Motif Analysis of CgGRASs
4.6. Collinearity and Chromosomal Localization of CgGRASs
4.7. Cis-elements and Gene Ontology Analysis of CgGRASs
4.8. RT-qPCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Huang, Y.; Zheng, Q.; Zhang, M.-M.; He, X.; Zhao, X.; Wang, L.; Lan, S.; Liu, Z.-J. Genome-Wide Identification and Expression Analysis of the GRAS Gene Family and Their Responses to Heat Stress in Cymbidium goeringii. Int. J. Mol. Sci. 2024, 25, 6363. https://doi.org/10.3390/ijms25126363
Huang Y, Zheng Q, Zhang M-M, He X, Zhao X, Wang L, Lan S, Liu Z-J. Genome-Wide Identification and Expression Analysis of the GRAS Gene Family and Their Responses to Heat Stress in Cymbidium goeringii. International Journal of Molecular Sciences. 2024; 25(12):6363. https://doi.org/10.3390/ijms25126363
Chicago/Turabian StyleHuang, Ye, Qinyao Zheng, Meng-Meng Zhang, Xin He, Xuewei Zhao, Linying Wang, Siren Lan, and Zhong-Jian Liu. 2024. "Genome-Wide Identification and Expression Analysis of the GRAS Gene Family and Their Responses to Heat Stress in Cymbidium goeringii" International Journal of Molecular Sciences 25, no. 12: 6363. https://doi.org/10.3390/ijms25126363