Effects of Different Doses of sUV-B Exposure on Taxane Compounds’ Metabolism in Taxus wallichiana var. Mairei
Abstract
:1. Introduction
2. Results
2.1. Effects of Different Doses of sUV-B Exposure on the Growth Biomass and Leaf Characteristics of Taxus wallichiana var. Mairei
2.2. Effect of Different Doses of sUV-B Exposure on the Endogenous Hormone Content of Taxus wallichiana var. Mairei
2.3. Effect of Different Doses of sUV-B Exposure on the Taxane Compound Contents of Taxus wallichiana var. Mairei
2.4. Effect of Different Doses of sUV-B Exposure on the Expression of Key Genes for Paclitaxel Synthesis
2.5. Correlation Analysis between Endogenous Hormone Content, Secondary Metabolite Content, and the Expression of Key Enzyme Genes for Paclitaxel Synthesis
2.6. Comprehensive Evaluation Based on Factor Analysis
3. Discussion
4. Materials and Methods
4.1. Plant Materials and sUV-B Exposure
4.2. Measurement of Growth Biomass and Physiological Characteristics of Taxus wallichiana var. Mairei
4.3. Measurement of the Endogenous Hormone Content of Taxus wallichiana var. Mairei
4.4. Measurement of the Specific Secondary Metabolite Contents of Taxus wallichiana var. Mairei
4.5. Measurement of Gene Expression of Key Enzymes in Paclitaxel Synthesis of Taxus wallichiana var. Mairei
4.6. Factor Analysis
4.7. Data Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | Plant Height (cm) | Leaf Length (cm) | Leaf Width (cm) | Leaf Area (cm2) | Leaf Thickness (mm) | Leaf Water Content (%) |
---|---|---|---|---|---|---|
CK | 49.93 ± 1.703 a | 9.7 ± 1.422 a | 5.4 ± 0.31 a | 25.77 ± 2.51 a | 0.11 ± 0.0057 a | 71.76 ± 0.741 ab |
T1 | 49.73 ± 1.43 a | 9.167 ± 0.89 a | 5.47 ± 0.145 a | 25.12 ± 2.8 a | 0.12 ± 0.0033 a | 70.39 ± 0.18 b |
T2 | 48.87 ± 0.25 a | 9.13 ± 0.98 a | 5.43 ± 0.425 a | 25.17 ± 4.46 a | 0.11 ± 0.001 a | 72.85 ± 0.283 a |
T3 | 48.03 ± 1.84 a | 9.17 ± 0.84 a | 4.63 ± 0.08 a | 21.21 ± 1.78 a | 0.09 ± 0.001 b | 69.02 ± 0.38 b |
Factor | Ingredient | ||
---|---|---|---|
1 | 2 | 3 | |
Q1 | 0.089 | 0.083 | −0.002 |
Q2 | −0.016 | 0.180 | 0.025 |
Q3 | 0.100 | −0.008 | 0.072 |
Q4 | 0.086 | 0.027 | 0.083 |
Q5 | 0.118 | −0.019 | 0.012 |
Q6 | 0.034 | −0.019 | 0.176 |
Q7 | −0.021 | −0.174 | 0.057 |
Q8 | 0.121 | −0.086 | 0.002 |
Q9 | −0.023 | 0.178 | 0.036 |
Q10 | 0.010 | 0.012 | 0.185 |
Q11 | 0.064 | −0.173 | 0.076 |
Q12 | −0.115 | 0.116 | 0.004 |
Q13 | 0.075 | −0.030 | −0.178 |
Q14 | 0.092 | 0.026 | −0.157 |
Q15 | 0.116 | 0.009 | −0.008 |
Q16 | 0.088 | 0.066 | −0.139 |
Q17 | 0.018 | −0.004 | 0.184 |
Q18 | 0.004 | 0.170 | 0.030 |
Q19 | 0.113 | 0.031 | −0.039 |
UV-B Treatment | F1 | F2 | F3 | F | Rank |
---|---|---|---|---|---|
CK | 0.04253 | −0.87442 | 1.21803 | 0.57 | 3 |
T1 | 1.14458 | −0.34742 | −0.90512 | 0.17 | 2 |
T2 | 0.10771 | 1.43804 | 0.41285 | 0.1 | 1 |
T3 | −1.29481 | −0.21621 | −0.72576 | −0.83 | 4 |
Primer | Primer Sequence | PCR Amplicon Size (bp) | Gene ID |
---|---|---|---|
18S-F | 5′-GTGCACATCCCGACTCT-3′ | 102 | AF259290.1 |
18S-R | 5′-GCGATCCGTCGAGTTATCAT-3′ | ||
CoA-ligase-F | 5′-AGCAGACACTATGGAACA-3′ | 109 | KM593667.1 |
CoA-ligase-R | 5′-GCCACAACTCTCCTCTAT-3′ | ||
Hmgr-F | 5′-TAGGGCTCCCGTTGTTAGGT-3′ | 207 | AY544991.1 |
Hmgr-R | 5′-TTCATCCCCATGGCATCTCC-3′ | ||
Ts-F | 5′-TTCGCACGCACGGATACG-3′ | 115 | AY007207.1 |
Ts-R | 5′-TTCACCACGCTTCTCAATTCG-3′ | ||
Dbat-F | 5′-AGTTGGATTTGGTGATCGACTT-3′ | 92 | AY365031.2 |
Dbat-R | 5′-ATCCATGTTGCACGAGACTT-3′ | ||
Bapt-F | 5′-TAAGCACTCTACAACAACAATGG-3′ | 111 | NC003071.7 |
Bapt-R | 5′-GCATGAACATTAGTATCTTGATTCC-3′ | ||
Dbtnbt-F | 5′-CGGGGGGTTTGTTGTGGGATTA-3′ | 104 | AY326950.1 |
Dbtnbt-R | 5′-TTAGCCTCTCCCCTCGCCATCT-3′ |
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Zhong, W.; Tian, X.; Zhang, Y.; Tang, X.; Xiao, S.; Zhang, Y.; Yang, J.; Liu, Y.; Li, D. Effects of Different Doses of sUV-B Exposure on Taxane Compounds’ Metabolism in Taxus wallichiana var. Mairei. Int. J. Mol. Sci. 2024, 25, 6407. https://doi.org/10.3390/ijms25126407
Zhong W, Tian X, Zhang Y, Tang X, Xiao S, Zhang Y, Yang J, Liu Y, Li D. Effects of Different Doses of sUV-B Exposure on Taxane Compounds’ Metabolism in Taxus wallichiana var. Mairei. International Journal of Molecular Sciences. 2024; 25(12):6407. https://doi.org/10.3390/ijms25126407
Chicago/Turabian StyleZhong, Weixue, Xuchen Tian, Ye Zhang, Xiaoqing Tang, Siqiu Xiao, Ying Zhang, Jing Yang, Ying Liu, and Dewen Li. 2024. "Effects of Different Doses of sUV-B Exposure on Taxane Compounds’ Metabolism in Taxus wallichiana var. Mairei" International Journal of Molecular Sciences 25, no. 12: 6407. https://doi.org/10.3390/ijms25126407