Residence of the Nucleotide Sugar Transporter Family Members SLC35F1 and SLC35F6 in the Endosomal/Lysosomal Pathway
Abstract
:1. Introduction
2. Results
2.1. Human SLC35F1 (hSLC35F1) and SLC35F6 (hSLC35F6) Have Different Main Subcellular Localizations in HeLa Cells
2.2. In SH-SY5Y Neuronal Cells, Endogenously Expressed SLC35F1 and SLC35F6 Are Located in Recycling Endosomes and Lysosomes, Respectively
2.3. Overexpression of Rab11 Disrupts the Intracellular Distribution of SLC35F1 but Has Little Effect on SLC35F6 Localization
2.4. The C-Terminal Tails of SLC35F1 and SLC35F6 Contain the Molecular Information Responsible for Their Differential Trafficking
2.5. Two YXXΦ Sorting Motifs in SLC35F1 and a [D/E]XXXL[L/I] Motif in SLC35F6 C-Terminal Tails Are Critical for Their Respective Trafficking
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. DNA Constructs
4.3. Cell Culture and Transient Transfection
4.4. Western Blotting
4.5. Immunofluorescence
4.6. Cell Surface Biotinylation Assay
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Van den Bossche, F.; Tevel, V.; Gilis, F.; Gaussin, J.-F.; Boonen, M.; Jadot, M. Residence of the Nucleotide Sugar Transporter Family Members SLC35F1 and SLC35F6 in the Endosomal/Lysosomal Pathway. Int. J. Mol. Sci. 2024, 25, 6718. https://doi.org/10.3390/ijms25126718
Van den Bossche F, Tevel V, Gilis F, Gaussin J-F, Boonen M, Jadot M. Residence of the Nucleotide Sugar Transporter Family Members SLC35F1 and SLC35F6 in the Endosomal/Lysosomal Pathway. International Journal of Molecular Sciences. 2024; 25(12):6718. https://doi.org/10.3390/ijms25126718
Chicago/Turabian StyleVan den Bossche, François, Virginie Tevel, Florentine Gilis, Jean-François Gaussin, Marielle Boonen, and Michel Jadot. 2024. "Residence of the Nucleotide Sugar Transporter Family Members SLC35F1 and SLC35F6 in the Endosomal/Lysosomal Pathway" International Journal of Molecular Sciences 25, no. 12: 6718. https://doi.org/10.3390/ijms25126718