Mode of Action of Antimicrobial Potential Protease SH21 Derived from Bacillus siamensis

Tarek, Hasan; Cho, Seung Sik; Nam, Kyung Bin; Lee, Ji Min; Lee, Sang Hun; Yoo, Jin Cheol

doi:10.3390/ijms25137046

Open AccessCommunication

Mode of Action of Antimicrobial Potential Protease SH21 Derived from Bacillus siamensis

by

Hasan Tarek

^1,†

,

Seung Sik Cho

^2,3,†

,

Kyung Bin Nam

¹,

Ji Min Lee

¹,

Sang Hun Lee

¹ and

Jin Cheol Yoo

^1,*

¹

Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea

²

Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan 58554, Republic of Korea

³

Department of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Muan 58554, Republic of Korea

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2024, 25(13), 7046; https://doi.org/10.3390/ijms25137046

Submission received: 24 April 2024 / Revised: 13 June 2024 / Accepted: 19 June 2024 / Published: 27 June 2024

(This article belongs to the Special Issue Natural Compounds: Advances in Antimicrobial Activity)

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Abstract

Global public health is facing a major issue with emerging resistance to antimicrobial agents. Antimicrobial agents that are currently on the market are strong and efficient, but it has not been ruled out that these medications will eventually cause resistance to bacteria. Exploring novel bioactive compounds derived from natural sources is therefore, crucial to meet future demands. The present study evaluated the mode of action of the antimicrobial potential protease enzyme SH21. Protease SH21 exhibited antimicrobial activity, strong heat stability (up to 100 °C), and pH stability (pH 3.0 to 9.0). In terms of mode of action, we found that protease SH21 was able to disrupt the bacterial cell membrane as the results of the nucleotide leakage and cell membrane permeability assay. In addition, we also checked inner membrane permeability by PI uptake assay which suggested that protease SH21 has the ability to enter the bacterial cell membrane. Our results revealed that the antimicrobial protease SH21 might be a promising candidate for treating microbial infections.

Keywords: protease SH21; antimicrobial agents; microbial infections; PI uptake; membrane permeability

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MDPI and ACS Style

Tarek, H.; Cho, S.S.; Nam, K.B.; Lee, J.M.; Lee, S.H.; Yoo, J.C. Mode of Action of Antimicrobial Potential Protease SH21 Derived from Bacillus siamensis. Int. J. Mol. Sci. 2024, 25, 7046. https://doi.org/10.3390/ijms25137046

AMA Style

Tarek H, Cho SS, Nam KB, Lee JM, Lee SH, Yoo JC. Mode of Action of Antimicrobial Potential Protease SH21 Derived from Bacillus siamensis. International Journal of Molecular Sciences. 2024; 25(13):7046. https://doi.org/10.3390/ijms25137046

Chicago/Turabian Style

Tarek, Hasan, Seung Sik Cho, Kyung Bin Nam, Ji Min Lee, Sang Hun Lee, and Jin Cheol Yoo. 2024. "Mode of Action of Antimicrobial Potential Protease SH21 Derived from Bacillus siamensis" International Journal of Molecular Sciences 25, no. 13: 7046. https://doi.org/10.3390/ijms25137046

APA Style

Tarek, H., Cho, S. S., Nam, K. B., Lee, J. M., Lee, S. H., & Yoo, J. C. (2024). Mode of Action of Antimicrobial Potential Protease SH21 Derived from Bacillus siamensis. International Journal of Molecular Sciences, 25(13), 7046. https://doi.org/10.3390/ijms25137046

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Mode of Action of Antimicrobial Potential Protease SH21 Derived from Bacillus siamensis

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