Netrin-1 Is an Important Mediator in Microglia Migration
Abstract
:1. Introduction
2. Results
2.1. Netrin-1 Promotes BV2 Elongation and Migration
2.2. Netrin-1 Increases GSK3β Activity by Suppressing GSK3β Phosphorylation at Serine 9
2.3. Netrin-1 Regulates BV2 Elongation and Migration through Activating GSK3β Activity
2.4. Integrin α6/β1 Is Highly Expressed in Microglia and Interacts with Netrin-1
2.5. Activating Integrin α6/β1 Mimics Netrin-1-Induced GSK3β Activity as well as BV2 Elongation and Migration
2.6. Netrin-1 Is Highly Expressed in the Developing Cerebral Cortex
2.7. Netrin-1 Knockout Suppresses Microglia Migration into the Cerebral Cortex during Early Brain Development
3. Discussion
4. Materials and Method
4.1. Animals
4.2. Reagents
4.3. Cell Culture and Collection of Netrin-1 Conditioned Medium
4.4. In Vitro Cell Migration Assay
4.5. Immunostaining
4.6. Immunoblotting
4.7. Microscale Thermophoresis (MST) Measurement
4.8. Imaging and Quantification
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yu, H.-L.; Liu, X.; Yin, Y.; Liu, X.-N.; Feng, Y.-Y.; Tahir, M.M.; Miao, X.-Z.; He, X.-X.; He, Z.-X.; Zhu, X.-J. Netrin-1 Is an Important Mediator in Microglia Migration. Int. J. Mol. Sci. 2024, 25, 7079. https://doi.org/10.3390/ijms25137079
Yu H-L, Liu X, Yin Y, Liu X-N, Feng Y-Y, Tahir MM, Miao X-Z, He X-X, He Z-X, Zhu X-J. Netrin-1 Is an Important Mediator in Microglia Migration. International Journal of Molecular Sciences. 2024; 25(13):7079. https://doi.org/10.3390/ijms25137079
Chicago/Turabian StyleYu, Hua-Li, Xiu Liu, Yue Yin, Xiao-Nuo Liu, Yu-Yao Feng, Muhammad Mateen Tahir, Xin-Zhi Miao, Xiao-Xiao He, Zi-Xuan He, and Xiao-Juan Zhu. 2024. "Netrin-1 Is an Important Mediator in Microglia Migration" International Journal of Molecular Sciences 25, no. 13: 7079. https://doi.org/10.3390/ijms25137079