Piezo1 Activation Drives Enhanced Collagen Synthesis in Aged Animal Skin Induced by Poly L-Lactic Acid Fillers
Abstract
:1. Introduction
2. Results
2.1. Poly L-lactic Acid Increases Intracellular Ca2+ Levels in Senescent Fibroblasts
2.2. Poly L-lactic Acid Enhances ERK1/2/AKT, CDK4, Cyclin D1, and Proliferation of Senescent Fibroblasts
2.3. Poly L-lactic Acid Upregulates mTOR/S6K1/4EBP1, TGF-β, and COLLAGEN I/III Expression in Senescent Fibroblasts
2.4. Poly L-lactic Acid Increases ERK1/2/AKT, CDK4, and Cyclin D1 Expression and Proliferation of Fibroblasts in Aged Skin
2.5. mTOR/S6K1/4EBP1, TGF-β, and Collagen I/III Expression Is Upregulated in Aged Skin
3. Discussion
4. Materials and Methods
4.1. Poly L-lactic Acid Preparation
4.2. In Vitro Experiments
4.2.1. Cell Culture
4.2.2. Experimental Design
4.3. In Vivo Experiments
4.3.1. Mouse Model and Maintenance
4.3.2. Experimental Design
4.4. Sample Preparation
4.4.1. Protein Isolation
4.4.2. Tissue Processing, Paraffin Embedding and Sectioning
4.5. Measurement of Intracellular Ca2+ Concentration
4.6. Western Blot Analysis
4.7. Enzyme-Linked Immunosorbent Assays
4.8. Staining
4.8.1. Immunohistochemistry
4.8.2. Masson Trichrome Staining
4.8.3. Herovici Staining
4.8.4. Quantitative Analysis and Procedure of Scanning and Image Selection
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Byun, K.-A.; Lee, J.H.; Lee, S.Y.; Oh, S.; Batsukh, S.; Cheon, G.-w.; Lee, D.; Hong, J.H.; Son, K.H.; Byun, K. Piezo1 Activation Drives Enhanced Collagen Synthesis in Aged Animal Skin Induced by Poly L-Lactic Acid Fillers. Int. J. Mol. Sci. 2024, 25, 7232. https://doi.org/10.3390/ijms25137232
Byun K-A, Lee JH, Lee SY, Oh S, Batsukh S, Cheon G-w, Lee D, Hong JH, Son KH, Byun K. Piezo1 Activation Drives Enhanced Collagen Synthesis in Aged Animal Skin Induced by Poly L-Lactic Acid Fillers. International Journal of Molecular Sciences. 2024; 25(13):7232. https://doi.org/10.3390/ijms25137232
Chicago/Turabian StyleByun, Kyung-A, Je Hyuk Lee, So Young Lee, Seyeon Oh, Sosorburam Batsukh, Gwahn-woo Cheon, Dongun Lee, Jeong Hee Hong, Kuk Hui Son, and Kyunghee Byun. 2024. "Piezo1 Activation Drives Enhanced Collagen Synthesis in Aged Animal Skin Induced by Poly L-Lactic Acid Fillers" International Journal of Molecular Sciences 25, no. 13: 7232. https://doi.org/10.3390/ijms25137232