PIEZO1 Promotes the Migration of Endothelial Cells via Enhancing CXCR4 Expression under Simulated Microgravity
Abstract
:1. Introduction
2. Results
2.1. Simulated Microgravity Increases the Expression of PIEZO1 in HUVECs
2.2. Simulated Microgravity Promotes Endothelial Cell Migration through PIEZO1
2.3. PIEZO1 Knock-Down in HUVECs after Exposure to Simulated Microgravity Results in Changes in Several Biological Processes
2.4. PIEZO1 Enhances the Expression of CXCR4 through Ca2+ Influx
2.5. CXCR4 Promotes the Migration of HUVECs under Simulated Microgravity
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Drug Treatment
4.2. Clinorotation to Simulate Microgravity
4.3. qRT-PCR Analysis
4.4. Western Blot Analysis
4.5. Immunofluorescence Staining
4.6. Transfection of Small Interfering RNA
4.7. Cytosolic Ca2+ Measurements
4.8. Transwell Assays
4.9. Wound-Healing Assay
4.10. Mass Spectrometric Analysis
4.10.1. Sample Preparation
4.10.2. Liquid Chromatography and Tandem Mass Spectrometry (LC–MS/MS)
4.10.3. Data Processing
4.10.4. Bioinformatics Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, Y.; Li, C.; Wang, R.; Zhao, X.; Pan, Y.; Zhang, Q.; Li, S.; Fan, J.; Wang, Y.; Sun, X. PIEZO1 Promotes the Migration of Endothelial Cells via Enhancing CXCR4 Expression under Simulated Microgravity. Int. J. Mol. Sci. 2024, 25, 7254. https://doi.org/10.3390/ijms25137254
Wang Y, Li C, Wang R, Zhao X, Pan Y, Zhang Q, Li S, Fan J, Wang Y, Sun X. PIEZO1 Promotes the Migration of Endothelial Cells via Enhancing CXCR4 Expression under Simulated Microgravity. International Journal of Molecular Sciences. 2024; 25(13):7254. https://doi.org/10.3390/ijms25137254
Chicago/Turabian StyleWang, Yuan, Chengfei Li, Ruonan Wang, Xingcheng Zhao, Yikai Pan, Qian Zhang, Shuhan Li, Jieyi Fan, Yongchun Wang, and Xiqing Sun. 2024. "PIEZO1 Promotes the Migration of Endothelial Cells via Enhancing CXCR4 Expression under Simulated Microgravity" International Journal of Molecular Sciences 25, no. 13: 7254. https://doi.org/10.3390/ijms25137254