Benzoxazinoids Biosynthetic Gene Cluster Identification and Expression Analysis in Maize under Biotic and Abiotic Stresses
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification and Chromosome Distribution of BGC Gene Members
2.2. Characterization and Subcellular Location of BGC Gene Members
2.3. Sequence Analysis and Classification of BGC Gene Members
2.4. Expression Patterns of BGC Gene Members under Multiple Biotic/Abiotic Stresses
2.5. Identification of Core Conserved BGC Gene Members in Maize
2.6. Effect of Multiple Stress Presences on Expression Levels of Core Conserved DEGs in BGC Genes and DIMBOA Accumulation
3. Discussion
4. Materials and Methods
4.1. Genome-Wide Identification and Mapping of BGC Gene Members in Maize
4.2. Sequence Analysis, Structural Characterization, Subcellular Localization, and Phylogenetic Tree of BGC Gene Members in Maize
4.3. Expression Patterns of BGC Gene Members in Different Abiotic/Biotic-Induced Tissues by RNA-Seq
4.4. Core Conserved BGC Gene Members Identification
4.5. qRT-PCR Analysis of Core Conserved BGC Gene Members
4.6. Quantification of DIMBOA Contents
4.7. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, X.; Shi, Z.; He, F.; Niu, Y.; Qi, G.; Sun, S.; Li, X.; Gao, X. Benzoxazinoids Biosynthetic Gene Cluster Identification and Expression Analysis in Maize under Biotic and Abiotic Stresses. Int. J. Mol. Sci. 2024, 25, 7460. https://doi.org/10.3390/ijms25137460
Zhao X, Shi Z, He F, Niu Y, Qi G, Sun S, Li X, Gao X. Benzoxazinoids Biosynthetic Gene Cluster Identification and Expression Analysis in Maize under Biotic and Abiotic Stresses. International Journal of Molecular Sciences. 2024; 25(13):7460. https://doi.org/10.3390/ijms25137460
Chicago/Turabian StyleZhao, Xiaoqiang, Zhenzhen Shi, Fuqiang He, Yining Niu, Guoxiang Qi, Siqi Sun, Xin Li, and Xiquan Gao. 2024. "Benzoxazinoids Biosynthetic Gene Cluster Identification and Expression Analysis in Maize under Biotic and Abiotic Stresses" International Journal of Molecular Sciences 25, no. 13: 7460. https://doi.org/10.3390/ijms25137460