Comparative Transcriptome Analysis of Henosepilachna vigintioctomaculata Reveals Critical Pathways during Development
Abstract
:1. Introduction
2. Results
2.1. Data Description and Processing
2.2. Function Annotation
2.3. Differential Expression Genes
2.4. Adult versus Egg
2.5. Egg versus Larvae
2.6. Larva versus Pupa
2.7. Adult versus Pupa
2.8. Adult versus Larva
2.9. Egg versus Pupa
3. Discussion
3.1. Potential Targets for Control throughout the Whole Lifespan
3.2. Targeting Energy Generation Genes in Embryonic and Pupal Stages
3.3. Antifeedants for Larvae and Adults
4. Materials and Methods
4.1. Insect Rearing
4.2. Sample Collection
4.3. Total RNA Isolation
4.4. Library Construction and Transcriptomic Sequencing
4.5. Sequencing Processing and Assembly
4.6. Identification of Differentially Expressed Genes
4.7. Transcript Data Annotation and Gene Ontology
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, Y.-X.; Wu, Y.-K.; Liu, H.-H.; Li, W.-Z.; Jin, L.; Li, G.-Q. Comparative Transcriptome Analysis of Henosepilachna vigintioctomaculata Reveals Critical Pathways during Development. Int. J. Mol. Sci. 2024, 25, 7505. https://doi.org/10.3390/ijms25147505
Zhang Y-X, Wu Y-K, Liu H-H, Li W-Z, Jin L, Li G-Q. Comparative Transcriptome Analysis of Henosepilachna vigintioctomaculata Reveals Critical Pathways during Development. International Journal of Molecular Sciences. 2024; 25(14):7505. https://doi.org/10.3390/ijms25147505
Chicago/Turabian StyleZhang, Yu-Xing, Yi-Kuan Wu, Hai-Hui Liu, Wen-Ze Li, Lin Jin, and Guo-Qing Li. 2024. "Comparative Transcriptome Analysis of Henosepilachna vigintioctomaculata Reveals Critical Pathways during Development" International Journal of Molecular Sciences 25, no. 14: 7505. https://doi.org/10.3390/ijms25147505