Transcriptome and Metabolome Analyses of Leaves from Cutting Rejuvenation of Ancient Cinnamomum camphora
Abstract
:1. Introduction
2. Results
2.1. The Leaf Phenotype and Tissue Structure of Ancient C. camphora and Its Cutting Plantlets
2.2. Analysis of Plant Hormone Contents in the Leaves of an Ancient C. camphora and Its Cutting Plantlets
2.3. Identification of DAMs in the Leaves of Ancient C. camphora and Its Cutting Plantlets
2.4. DEG Identification and Analysis in the Leaves of an Ancient C. camphora and Its Cutting Plantlets
2.5. The Sequence Data Conjoint Analysis of DEGs and DAMs
2.6. qRT–PCR Verification of DEGs
3. Discussion
3.1. Leaf Differences between Juvenile and Mature Plants
3.2. Plant Hormone Contents and Gene Expression Regulatory Function
3.3. Identification of Differential Accumulated Primary Metabolites
3.4. Gene Identification of Metabolic Pathways Related to Stress Resistance
4. Materials and Methods
4.1. Plant Materials and Cutting Method
4.2. Leaf Cytological Structural Observation
4.3. Metabolome Analysis
4.3.1. Plant Hormone Metabolome Sequencing and Analysis
4.3.2. Primary Metabolome Sequencing and Analysis
4.4. RNA Extraction and Transcriptome Sequencing
4.5. Quantitative Real-Time PCR (qRT–PCR) Analysis
4.6. Data Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group Name | All Significant Difference | Down-Regulated | Up-Regulated |
---|---|---|---|
ML vs. RL | 235 | 89 | 146 |
YL vs. RL | 111 | 62 | 49 |
ML vs. YL | 92 | 38 | 54 |
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Liu, L.; Yang, A.; Liu, T.; Liu, S.; Hu, P.; Chen, C.; Zhou, H.; Wu, J.; Yu, F. Transcriptome and Metabolome Analyses of Leaves from Cutting Rejuvenation of Ancient Cinnamomum camphora. Int. J. Mol. Sci. 2024, 25, 7664. https://doi.org/10.3390/ijms25147664
Liu L, Yang A, Liu T, Liu S, Hu P, Chen C, Zhou H, Wu J, Yu F. Transcriptome and Metabolome Analyses of Leaves from Cutting Rejuvenation of Ancient Cinnamomum camphora. International Journal of Molecular Sciences. 2024; 25(14):7664. https://doi.org/10.3390/ijms25147664
Chicago/Turabian StyleLiu, Lipan, Aihong Yang, Tengyun Liu, Shujuan Liu, Ping Hu, Caihui Chen, Hua Zhou, Jingfang Wu, and Faxin Yu. 2024. "Transcriptome and Metabolome Analyses of Leaves from Cutting Rejuvenation of Ancient Cinnamomum camphora" International Journal of Molecular Sciences 25, no. 14: 7664. https://doi.org/10.3390/ijms25147664