Antioxidant Activity and the Therapeutic Effect of Sinomenine Hydrochloride-Loaded Liposomes-in-Hydrogel on Atopic Dermatitis
Abstract
:1. Introduction
2. Results
2.1. Molecular Docking of SIN as the Active Structure of SINH
2.2. Basic Characteristics of SINH-L-H
2.3. In Vitro/Ex Vivo Dialysis Diffusion Dialysis
2.4. Determination of Antioxidant Activity
2.4.1. The Scavenging Rate on Hydroxyl Radicals
2.4.2. The Inhibitory Rate on ABTS Radicals
2.5. The Therapeutic Effects on AD Mice
2.5.1. Measurements Results of Skin Irritation of SINH-L-H
2.5.2. General Condition of Mice and Manifestation of Skin Lesions
2.5.3. Ear Swelling in Mice
2.5.4. Mouse Organ Index
2.5.5. Malondialdehyde (MDA) in Mouse Organ
2.5.6. HE Staining
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Animals
4.3. Preparation SINH-L, SINH-H, and SINH-L-H
4.4. Quality Evaluation of SINH-L
4.4.1. Appearance and Morphology of Cryo-EM
4.4.2. Particle Size and Zeta Potential
4.4.3. Encapsulation Rate and Drug Load
4.5. Molecular Docking
4.6. Ex Vivo/Ex Vivo Dialysis Diffusion Dialysis of SH-L-H
4.7. Determination of Antioxidant Activity of SINH-L-H
4.7.1. The Scavenging Rate on Hydroxyl Radicals
4.7.2. The Inhibitory Rate on ABTS Radicals
4.8. The Therapeutic Effect of SINH-L-H on AD
4.8.1. Skin Irritation of SINH-L-H
4.8.2. Establishment of AD Model and Administration
4.8.3. Degree of Skin Lesion Score (EASI Score) in AD Mice
4.8.4. Determination of Ear Swelling Rate in Mice
4.8.5. Measurement of Organ Index in Mice
4.8.6. Determination of MDA Content in Organ
4.8.7. Hematoxylin and Eosin Staining
4.8.8. Statistical Methods
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Storage Temperature | Storage Period (d) | Characters | Exterior | Viscosity (_mPa·s) |
---|---|---|---|---|
4 °C | 0 | Milky white, even and delicate | 9.84 ± 0.14 | |
7 | Milky white, even and delicate | 9.76 ± 0.08 | ||
14 | Milky white, even and delicate | 9.80 ± 0.03 | ||
21 | Milky white, even and delicate | 9.79 ± 0.05 | ||
30 | Milky white, even and delicate | 9.82 ± 0.03 | ||
Normal temperature | 0 | Milky white, even and delicate | 9.84 ± 0.02 | |
7 | Milky white, even and delicate | 9.73 ± 0.01 | ||
14 | Milky white, even and delicate | 9.70 ± 0.03 | ||
21 | Pale yellow, even and delicate | 9.65 ± 0.04 | ||
30 | Yellow, layered | 9.57 ± 0.06 |
Time | Group | Irritation Intensity | |||
---|---|---|---|---|---|
B-L-H Skin Status | B-L-H Skin Score | SINH-L-H Skin Status | SINH-L-H Skin Score | ||
1 h | 0 | 0 | None | ||
24 h | 0 | 0.2 | None | ||
48 h | 0 | 0 | None | ||
72 h | 0 | 0 | None |
Time | Group | Irritation Intensity | |||
---|---|---|---|---|---|
B-L-H Skin Status | B-L-H Skin Score | SINH-L-H Skin Status | SINH-L-H Skin Score | ||
1 d | 0 | 0 | None | ||
2 d | 0 | 0.2 | None | ||
3 d | 0 | 0 | None | ||
4 d | 0 | 0 | None | ||
5 d | 0 | 0 | None | ||
6 d | 0 | 0 | None | ||
7 d | 0 | 0 | None |
Skin Reaction | Degree of Response | Score |
---|---|---|
Erythema and eschar | Erythema free | 0 |
Slight erythema (barely visible) | 1 | |
Moderate erythema | 2 | |
Erythema severa | 3 | |
Purplish red erythema to slight eschar formation | 4 | |
Edema condition | No edema | 0 |
Slight edema (barely visible) | 1 | |
Mild edema (edema bulge clearly defined) | 2 | |
Moderate edema (edema bulge about 1 mm) | 3 | |
Severe edema (swelling of more than 1 mm, extended range) | 4 | |
Highest score | 8 |
Average Score | Irritation Intensity |
---|---|
0–0.49 | Non-irritant |
0.5–2.99 | Mild irritation |
3–5.99 | Moderate irritation |
6–8 | Strong irritation |
Skin Condition | Degree of Response | Points |
---|---|---|
No obvious skin injury was observed by naked eye | None | 0 |
Visual inspection requires careful examination to see the lesions | Mild | 1 |
The lesions are clearly visible to the naked eye | Medium | 2 |
The lesions were severe and very visible | Severe | 3 |
The score between the various symptoms can be recorded as a half grade (0.5) |
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Chen, X.; Wu, Y.; Jia, R.; Fang, Y.; Cao, K.; Yang, X.; Qu, X.; Xia, H. Antioxidant Activity and the Therapeutic Effect of Sinomenine Hydrochloride-Loaded Liposomes-in-Hydrogel on Atopic Dermatitis. Int. J. Mol. Sci. 2024, 25, 7676. https://doi.org/10.3390/ijms25147676
Chen X, Wu Y, Jia R, Fang Y, Cao K, Yang X, Qu X, Xia H. Antioxidant Activity and the Therapeutic Effect of Sinomenine Hydrochloride-Loaded Liposomes-in-Hydrogel on Atopic Dermatitis. International Journal of Molecular Sciences. 2024; 25(14):7676. https://doi.org/10.3390/ijms25147676
Chicago/Turabian StyleChen, Xue, Yang Wu, Ruoyang Jia, Yuqing Fang, Keang Cao, Xinying Yang, Xiaobo Qu, and Hongmei Xia. 2024. "Antioxidant Activity and the Therapeutic Effect of Sinomenine Hydrochloride-Loaded Liposomes-in-Hydrogel on Atopic Dermatitis" International Journal of Molecular Sciences 25, no. 14: 7676. https://doi.org/10.3390/ijms25147676