Abstract
Sensitive detection and efficient inactivation of pathogenic bacteria are crucial for halting the spread and reproduction of foodborne pathogenic bacteria. Herein, a novel Apt-modified PDMS-ZnO/Ag multifunctional biosensor has been developed for high-sensitivity surface-enhanced Raman scattering (SERS) detection along with photocatalytic sterilization towards Salmonella typhimurium (S. typhimurium). The distribution of the electric field in PDMS-ZnO/Ag with different Ag sputtering times was analyzed using a finite-difference time-domain (FDTD) algorithm. Due to the combined effect of electromagnetic enhancement and chemical enhancement, PDMS-ZnO/Ag exhibited outstanding SERS sensitivity. The limit of detection (LOD) for 4-MBA on the optimal SERS substrate (PZA-40) could be as little as 10−9 M. After PZA-40 was modified with the aptamer, the LOD of the PZA-40-Apt biosensor for detecting S. typhimurium was only 10 cfu/mL. Additionally, the PZA-40-Apt biosensor could effectively inactivate S. typhimurium under visible light irradiation within 10 min, with a bacterial lethality rate (Lb) of up to 97%. In particular, the PZA-40-Apt biosensor could identify S. typhimurium in food samples in addition to having minimal cytotoxicity and powerful biocompatibility. This work provides a multifunctional nanoplatform with broad prospects for selective SERS detection and photocatalytic sterilization of pathogenic bacteria.
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MDPI and ACS Style
Qian, S.; Zhao, W.; Guo, R.; Wang, X.; Dai, H.; Lang, J.; Kadasala, N.R.; Jiang, Y.; Liu, Y.
Apt-Conjugated PDMS-ZnO/Ag-Based Multifunctional Integrated Superhydrophobic Biosensor with High SERS Activity and Photocatalytic Sterilization Performance. Int. J. Mol. Sci. 2024, 25, 7675.
https://doi.org/10.3390/ijms25147675
AMA Style
Qian S, Zhao W, Guo R, Wang X, Dai H, Lang J, Kadasala NR, Jiang Y, Liu Y.
Apt-Conjugated PDMS-ZnO/Ag-Based Multifunctional Integrated Superhydrophobic Biosensor with High SERS Activity and Photocatalytic Sterilization Performance. International Journal of Molecular Sciences. 2024; 25(14):7675.
https://doi.org/10.3390/ijms25147675
Chicago/Turabian Style
Qian, Sihan, Wenshi Zhao, Rui Guo, Xiaohan Wang, Huasong Dai, Jihui Lang, Naveen Reddy Kadasala, Yuhong Jiang, and Yang Liu.
2024. "Apt-Conjugated PDMS-ZnO/Ag-Based Multifunctional Integrated Superhydrophobic Biosensor with High SERS Activity and Photocatalytic Sterilization Performance" International Journal of Molecular Sciences 25, no. 14: 7675.
https://doi.org/10.3390/ijms25147675
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