Dual Role of microRNA-146a in Experimental Inflammation in Human Pulmonary Epithelial and Immune Cells and Expression in Inflammatory Lung Diseases
Abstract
:1. Introduction
2. Results
2.1. In Vitro Stimulation of Lung Epithelial Cells, M1 Macrophages, and Neutrophil-Precursor Cells Induces Activation of NF-kB Pathway
2.2. miR-146a-5p Is the Most Exclusively Upregulated miRNA in Stimulated A549, THP1, and HL60 Cells
2.3. Transfection with miR-146a-5p Mimic as Well as Anti-miR-146a-3p Results in Cell-Specific Downregulation of IL-8 Expression Levels
2.4. Modification of miR-146a-5p and miR-146a-3p Significantly Impacts IL-8 mRNA and Protein Expression in A549 Cells
2.5. Target Identification Analysis of miR-146a-5p and miR-146a-3p
2.6. miR-146a-5p Mimic Impacts miR-146a-3p Expression in Lung Epithelial Cells and in M1 Macrophages In Vitro
2.7. Expression of miR-146a-5p and miR-146a-3p Is Dysregulated in Inflammatory Lung Diseases
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Stimulation of Cells
4.2. miRNA Transfection
4.3. miRNA Isolation, Reverse Transcription, and Validation by qPCR
4.4. Reverse Transcription and qPCR of RNA Samples
4.5. Library Preparation, NGS, and Data Analysis
4.6. Flow Cytometry
4.7. Cytometric Bead Array
4.8. Protein Isolation and Western Blot
4.9. Patients and miRNA Expression
4.10. Pathway Enrichment Analysis of Target Genes
4.11. Data Analysis and Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gronau, L.; Duecker, R.P.; Jerkic, S.-P.; Eickmeier, O.; Trischler, J.; Chiocchetti, A.G.; Blumchen, K.; Zielen, S.; Schubert, R. Dual Role of microRNA-146a in Experimental Inflammation in Human Pulmonary Epithelial and Immune Cells and Expression in Inflammatory Lung Diseases. Int. J. Mol. Sci. 2024, 25, 7686. https://doi.org/10.3390/ijms25147686
Gronau L, Duecker RP, Jerkic S-P, Eickmeier O, Trischler J, Chiocchetti AG, Blumchen K, Zielen S, Schubert R. Dual Role of microRNA-146a in Experimental Inflammation in Human Pulmonary Epithelial and Immune Cells and Expression in Inflammatory Lung Diseases. International Journal of Molecular Sciences. 2024; 25(14):7686. https://doi.org/10.3390/ijms25147686
Chicago/Turabian StyleGronau, Lucia, Ruth P. Duecker, Silvija-Pera Jerkic, Olaf Eickmeier, Jordis Trischler, Andreas G. Chiocchetti, Katharina Blumchen, Stefan Zielen, and Ralf Schubert. 2024. "Dual Role of microRNA-146a in Experimental Inflammation in Human Pulmonary Epithelial and Immune Cells and Expression in Inflammatory Lung Diseases" International Journal of Molecular Sciences 25, no. 14: 7686. https://doi.org/10.3390/ijms25147686