Chemical Coaxing of Mesenchymal Stromal Cells by Drug Repositioning for Nestin Induction
Abstract
:1. Introduction
2. Results
2.1. Chemical Repositioning of Clinically Approved Drugs for Nestin Induction
2.2. Cellular Changes in Chemically Engineered Nestin-Expressing MSCs
2.3. Niche Cell Activity Induced by Vorinostat
3. Discussion
4. Material and Methods
4.1. Cell Culture
4.2. Treatment of Chemicals
4.3. RNA Extraction and Quantitative RT-PCR
4.4. Co-Culture
4.5. Neural Differentiation
4.6. Immunocytochemistry and Western Blotting
4.7. Flow Cytometry of Leukemic Cells and Mesenchymal Stromal Cells
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lim, S.-U.; Lee, D.-W.; Kim, J.-H.; Kang, Y.-J.; Kim, I.-Y.; Oh, I.-H. Chemical Coaxing of Mesenchymal Stromal Cells by Drug Repositioning for Nestin Induction. Int. J. Mol. Sci. 2024, 25, 8006. https://doi.org/10.3390/ijms25158006
Lim S-U, Lee D-W, Kim J-H, Kang Y-J, Kim I-Y, Oh I-H. Chemical Coaxing of Mesenchymal Stromal Cells by Drug Repositioning for Nestin Induction. International Journal of Molecular Sciences. 2024; 25(15):8006. https://doi.org/10.3390/ijms25158006
Chicago/Turabian StyleLim, Sun-Ung, Dae-Won Lee, Jung-Ho Kim, Young-Ju Kang, In-Yong Kim, and Il-Hoan Oh. 2024. "Chemical Coaxing of Mesenchymal Stromal Cells by Drug Repositioning for Nestin Induction" International Journal of Molecular Sciences 25, no. 15: 8006. https://doi.org/10.3390/ijms25158006