Comparison of Double-Stranded DNA at the 5′ and 3′ Ends of the G-Triplex and Its Application in the Detection of Hg(II)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Principles of Detecting Hg(II)
2.2. Effect of 5′/3′-dsDNA on the Fluorescence Signal of G3/ThT
2.3. Feasibility of G3MB6/ThT for Monitoring Hg(II)
2.4. Sensitivity and Selectivity of G3MB6/ThT for Detecting Hg(II)
2.5. Analysis of Hg(II) in Tap Water and Milk
3. Materials and Methods
3.1. Chemicals and Apparatus
3.2. Fluorescence of the G-Triplex with the Adjacent dsDNA
3.3. Fluorescence of Hg(II)
3.4. Detection of Hg(II) in Natural Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Intercalator | Linear Range (μM) | LOD (nM) | References |
---|---|---|---|
DAPI + NMM | 0–20 | 300 | [27] |
DAPI | 0–1.4 | 2.87 | [16] |
NMM | 0–1 | 18.6 | [28] |
Berberine | 0–10 | 7.7 | [29] |
Genefinder | 0–0.3 | 2.5 | [30] |
ThT | 0–4 | 20 | [31] |
ThT | 0–0.3 | 5.32 | This work |
Sample | Group | Added (nM) | Repeats | Found (nM) | Recovery (%) | Avg. (nM) |
---|---|---|---|---|---|---|
Milk | 1 | 19.24 | ||||
No.1 | 20 | 2 | 19.24 | 100.30 | 20.061 | |
3 | 21.70 | |||||
1 | 102.11 | |||||
No.2 | 100 | 2 | 102.93 | 103.20 | 103.203 | |
3 | 104.57 | |||||
1 | 253.08 | |||||
No.3 | 250 | 2 | 255.54 | 101.12 | 252.803 | |
3 | 249.79 | |||||
Water | 1 | 19.24 | ||||
No.1 | 20 | 2 | 20.06 | 101.67 | 20.334 | |
3 | 21.70 | |||||
1 | 102.93 | |||||
No.2 | 100 | 2 | 103.75 | 103.75 | 103.750 | |
3 | 104.57 | |||||
1 | 254.72 | |||||
No.3 | 250 | 2 | 256.36 | 102.54 | 256.359 | |
3 | 258.00 |
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Cai, Y.; Wu, Z.; Li, X.; Hu, X.; Wu, J.; You, Z.; Qiu, J. Comparison of Double-Stranded DNA at the 5′ and 3′ Ends of the G-Triplex and Its Application in the Detection of Hg(II). Int. J. Mol. Sci. 2024, 25, 8159. https://doi.org/10.3390/ijms25158159
Cai Y, Wu Z, Li X, Hu X, Wu J, You Z, Qiu J. Comparison of Double-Stranded DNA at the 5′ and 3′ Ends of the G-Triplex and Its Application in the Detection of Hg(II). International Journal of Molecular Sciences. 2024; 25(15):8159. https://doi.org/10.3390/ijms25158159
Chicago/Turabian StyleCai, Yule, Ziyi Wu, Xiangxiang Li, Xingting Hu, Jiamin Wu, Zhengying You, and Jieqiong Qiu. 2024. "Comparison of Double-Stranded DNA at the 5′ and 3′ Ends of the G-Triplex and Its Application in the Detection of Hg(II)" International Journal of Molecular Sciences 25, no. 15: 8159. https://doi.org/10.3390/ijms25158159