Identification and Analysis of the Mechanism of Stem Mechanical Strength Enhancement for Maize Inbred Lines QY1
Abstract
:1. Introduction
2. Results
2.1. Enhanced Mechanical Strength in Maize Inbred Line QY1
2.2. Increased Cell Wall Thickness in QY1 Stem Vascular Bundles
2.3. Increased Cellulose Content and Decreased Lignin Content in QY1 Stalks
2.4. Increased Saccharification Efficiency in Inbred Line QY1
2.5. QY1 Influences Stem Strength through Gene Regulation
2.6. Changes in Pectin Methylation and Methylesterase Activity in QY1
3. Discussion
3.1. Increased Cell Wall Thickness in QY1 Enhances Mechanical Strength of Stalks
3.2. Significant Changes in QY1 Cell Wall Components
3.3. Application of QY1 to Agricultural Production
4. Materials and Methods
4.1. Experimental Material
4.2. Determination of Plant Morphology and Mechanics
4.3. Microscopy Analysis
4.4. Phloroglucinol–HCl Stain
4.5. Extraction of Alcohol-Insoluble Residue
4.6. Determination of Cellulose and Lignin Content
4.7. Determination of Pectin Methylesterase (PME) Activity
4.8. Determination of the Degree of Pectin Methylesterification (DME)
4.9. Matrix Polysaccharide Composition Analysis
4.10. Determination of Cell Wall Saccharification Efficiency
4.11. Transcriptome Analysis
4.12. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Number | Comment | log2 Fold Change |
---|---|---|
Zm00001eb293700 | pectin methylesterase37 | 4.381 |
Zm00001eb130500 | pectin methylesterase19 | 3.458 |
Zm00001eb187870 | xyloglucan:xyloglucosyl transferase activity | 3.421 |
Zm00001eb330500 | pectin methylesterase20 | 3.294 |
Zm00001eb226490 | xyloglucan endo-transglycosylase/hydrolase4 | 2.558 |
Zm00001eb112510 | pectin methylesterase12 | 1.612 |
Zm00001eb353540 | pectin methylesterase38 | 1.591 |
Zm00001eb197870 | xyloglucan endo-transglycosylase/hydrolase6 | 1.348 |
Zm00001eb177120 | pectin methylesterase15 | 1.278 |
Zm00001eb411910 | xyloglucan endo-transglycosylase/hydrolase2 | 1.238 |
Zm00001eb341630 | xyloglucan:xyloglucosyl transferase activity | 1.071 |
Zm00001eb190890 | −2.232 | |
Zm00001eb312000 | xyloglucan:xyloglucosyl transferase activity | −2.363 |
Zm00001eb414360 | xyloglucan endo-transglycosylase/hydrolase7 | −2.501 |
Zm00001eb000660 | ring finger and WD40 repeat3 | −2.699 |
Zm00001eb327190 | pectin methylesterase41 | −4.286 |
Zm00001eb203620 | xyloglucan:xyloglucosyl transferase activity | −5.282 |
Zm00001eb414390 | xyloglucan endo-transglycosylase/hydrolase1 | −6.566 |
Gene Number | Comment | log2 Fold Change |
---|---|---|
Zm00001eb097410 | CesA3 | 7.88167584 |
Zm00001eb349960 | CesA3 | 7.74899559 |
Zm00001eb248410 | CesA2 | 4.276134325 |
Zm00001eb046440 | CesA10 | −1.139029969 |
Zm00001eb097100 | CesA6 | −1.99327018 |
Zm00001eb370930 | CesA14 | −2.746385239 |
Zm00001eb370940 | CesA8 | −3.092633023 |
Zm00001eb043490 | cellulose synthase (UDP-forming) activity | −5.400036017 |
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Yang, Y.; Mu, J.; Hao, X.; Yang, K.; Cao, Z.; Feng, J.; Li, R.; Zhang, N.; Zhou, G.; Kong, Y.; et al. Identification and Analysis of the Mechanism of Stem Mechanical Strength Enhancement for Maize Inbred Lines QY1. Int. J. Mol. Sci. 2024, 25, 8195. https://doi.org/10.3390/ijms25158195
Yang Y, Mu J, Hao X, Yang K, Cao Z, Feng J, Li R, Zhang N, Zhou G, Kong Y, et al. Identification and Analysis of the Mechanism of Stem Mechanical Strength Enhancement for Maize Inbred Lines QY1. International Journal of Molecular Sciences. 2024; 25(15):8195. https://doi.org/10.3390/ijms25158195
Chicago/Turabian StyleYang, Yumeng, Jianing Mu, Xiaoning Hao, Kangkang Yang, Ziyu Cao, Jiping Feng, Runhao Li, Ning Zhang, Gongke Zhou, Yingzhen Kong, and et al. 2024. "Identification and Analysis of the Mechanism of Stem Mechanical Strength Enhancement for Maize Inbred Lines QY1" International Journal of Molecular Sciences 25, no. 15: 8195. https://doi.org/10.3390/ijms25158195