Insulin-Activated Signaling Pathway and GLUT4 Membrane Translocation in hiPSC-Derived Cardiomyocytes
Abstract
:1. Introduction
2. Results
2.1. HiPSC-CMs Express Key Components of the Insulin-Activated Pathway
2.2. Insulin-Stimulated GLUT4 Membrane Translocation Is Visible Only on Membrane Lysates
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Line
4.3. Differentiation Protocol and Treatment
4.4. Total Lysates
4.5. Isolation of Membrane Lysates
4.6. Immunoblotting
4.7. Immunofluorescence
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Querio, G.; Antoniotti, S.; Levi, R.; Fleischmann, B.K.; Gallo, M.P.; Malan, D. Insulin-Activated Signaling Pathway and GLUT4 Membrane Translocation in hiPSC-Derived Cardiomyocytes. Int. J. Mol. Sci. 2024, 25, 8197. https://doi.org/10.3390/ijms25158197
Querio G, Antoniotti S, Levi R, Fleischmann BK, Gallo MP, Malan D. Insulin-Activated Signaling Pathway and GLUT4 Membrane Translocation in hiPSC-Derived Cardiomyocytes. International Journal of Molecular Sciences. 2024; 25(15):8197. https://doi.org/10.3390/ijms25158197
Chicago/Turabian StyleQuerio, Giulia, Susanna Antoniotti, Renzo Levi, Bernd K. Fleischmann, Maria Pia Gallo, and Daniela Malan. 2024. "Insulin-Activated Signaling Pathway and GLUT4 Membrane Translocation in hiPSC-Derived Cardiomyocytes" International Journal of Molecular Sciences 25, no. 15: 8197. https://doi.org/10.3390/ijms25158197