Dynamics of Replication-Associated Protein Levels through the Cell Cycle
Abstract
:1. Introduction
2. Results and Discussion
2.1. Double-Tagged Cell Lines for the Study of Cell Cycle-Dependent Expression at the Single-Cell Level
2.2. Sobel-Adjusted PCNA Fluorescence Intensity as an Indicator of Early, Middle, and Late S Phase
2.3. RIF1
2.4. MCM6
2.5. ORC1
2.6. Claspin
3. Materials and Methods
3.1. Cell Lines and Culture
3.2. Image Acquisition
3.3. Image Analysis and Cell Cycle Normalization
3.4. Software
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Atemin, A.; Ivanova, A.; Kanev, P.-B.; Uzunova, S.; Nedelcheva-Veleva, M.; Stoynov, S. Dynamics of Replication-Associated Protein Levels through the Cell Cycle. Int. J. Mol. Sci. 2024, 25, 8230. https://doi.org/10.3390/ijms25158230
Atemin A, Ivanova A, Kanev P-B, Uzunova S, Nedelcheva-Veleva M, Stoynov S. Dynamics of Replication-Associated Protein Levels through the Cell Cycle. International Journal of Molecular Sciences. 2024; 25(15):8230. https://doi.org/10.3390/ijms25158230
Chicago/Turabian StyleAtemin, Aleksandar, Aneliya Ivanova, Petar-Bogomil Kanev, Sonya Uzunova, Marina Nedelcheva-Veleva, and Stoyno Stoynov. 2024. "Dynamics of Replication-Associated Protein Levels through the Cell Cycle" International Journal of Molecular Sciences 25, no. 15: 8230. https://doi.org/10.3390/ijms25158230