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Open AccessArticle
Paeoniflorin Promotes Ovarian Development in Mice by Activating Mitophagy and Preventing Oxidative Stress
by
Huaming Xi
Huaming Xi †,
Ziqian Wang
Ziqian Wang †,
Minghui Li
Minghui Li ,
Xing Duan
Xing Duan * and
Yuan Li
Yuan Li *
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(15), 8355; https://doi.org/10.3390/ijms25158355 (registering DOI)
Submission received: 3 July 2024
/
Revised: 22 July 2024
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Accepted: 24 July 2024
/
Published: 30 July 2024
Abstract
During the development of animal organs, various adverse stimuli or toxic environments can induce oxidative stress and delay ovarian development. Paeoniflorin (PF), the main active ingredient of the traditional Chinese herb Paeonia lactiflora Pall., has protective effects on various diseases by preventing oxidative stress. However, the mechanism by which PF attenuates oxidative damage in mouse ovaries remains unclear. We evaluated the protective effects of PF on ovaries in an H2O2-induced mouse oxidative stress model. The H2O2-induced mouse ovarian oxidative stress model was used to explore the protective effect of PF on ovarian development. Histology and follicular development were observed. We then detected related indicators of cell apoptosis, oxidative stress, and autophagy in mouse ovaries. We found that PF inhibited H2O2-induced ovarian cell apoptosis and ferroptosis and promoted granulosa cell proliferation. PF prevented oxidative stress by increasing nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression levels. In addition, the autophagic flux of ovarian cells was activated and was accompanied by increased lysosomal biogenesis. Moreover, PF-mediated autophagy was involved in clearing mitochondria damaged by H2O2. Importantly, PF administration significantly increased the number of primordial follicles, primary follicles, secondary follicles, and antral follicles. PF administration improved ovarian sizes compared with the H2O2 group. The present study suggested that PF administration reversed H2O2-induced ovarian developmental delay and promoted follicle development. PF-activated mitophagy is crucial for preventing oxidative stress and improving mitochondrial quality.
Share and Cite
MDPI and ACS Style
Xi, H.; Wang, Z.; Li, M.; Duan, X.; Li, Y.
Paeoniflorin Promotes Ovarian Development in Mice by Activating Mitophagy and Preventing Oxidative Stress. Int. J. Mol. Sci. 2024, 25, 8355.
https://doi.org/10.3390/ijms25158355
AMA Style
Xi H, Wang Z, Li M, Duan X, Li Y.
Paeoniflorin Promotes Ovarian Development in Mice by Activating Mitophagy and Preventing Oxidative Stress. International Journal of Molecular Sciences. 2024; 25(15):8355.
https://doi.org/10.3390/ijms25158355
Chicago/Turabian Style
Xi, Huaming, Ziqian Wang, Minghui Li, Xing Duan, and Yuan Li.
2024. "Paeoniflorin Promotes Ovarian Development in Mice by Activating Mitophagy and Preventing Oxidative Stress" International Journal of Molecular Sciences 25, no. 15: 8355.
https://doi.org/10.3390/ijms25158355
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