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Open AccessArticle
Activity of N-Chlorotaurine against Periodontal Pathogens
by
Kacper Kowalczyk
Kacper Kowalczyk 1,
Débora C. Coraça-Huber
Débora C. Coraça-Huber 2
,
Walter Wille-Kollmar
Walter Wille-Kollmar 3,
Michael Berktold
Michael Berktold 1 and
Markus Nagl
Markus Nagl 1,*
1
Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, A-6020 Innsbruck, Austria
2
Research Laboratory for Biofilms and Implant Associated Infections (BIOFILM LAB), University Hospital for Orthopaedics and Traumatology, Medical University of Innsbruck, A-6020 Innsbruck, Austria
3
Independent Researcher, Dentist’s Practice, A-6020 Innsbruck, Austria
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(15), 8357; https://doi.org/10.3390/ijms25158357 (registering DOI)
Submission received: 27 June 2024
/
Revised: 24 July 2024
/
Accepted: 29 July 2024
/
Published: 30 July 2024
Abstract
Dental plaque bacteria play an important role in the pathogenicity of periodontitis and peri-implantitis. Therefore, antimicrobial agents are one means of treatment. N-chlorotaurine (NCT) as an endogenous well-tolerated topical antiseptic could be of advantage for this purpose. Accordingly, its microbicidal activity against some dental plaque bacteria was investigated at therapeutic concentrations in vitro. In quantitative killing assays, the activity of NCT against planktonic bacteria and against biofilms grown for 48 h on implantation screws was tested. Electron microscopy was used to demonstrate the formation of biofilm and its morphological changes. The killing of planktonic bacteria of all tested species, namely Streptococcus sanguinis, Streptococcus salivarius, Streptococcus oralis, Streptococcus cristatus, Rothia aeria, and Capnocytophaga ochracea, was shown within 10–20 min by 1% NCT in 0.01 M phosphate-buffered saline at 37 °C. Bacteria grown on screws for 24 h were inactivated by 1% NCT after 15–20 min as well, but the formation of biofilm on the screws was visible in electron microscopy not before 48 h. The killing of biofilms by 1% NCT was demonstrated after 30 min (streptococci) and 40 min (R. aeria). As expected, NCT has broad activity against dental plaque bacteria as well and should be further investigated on its clinical efficacy in periodontitis and peri-implantitis.
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MDPI and ACS Style
Kowalczyk, K.; Coraça-Huber, D.C.; Wille-Kollmar, W.; Berktold, M.; Nagl, M.
Activity of N-Chlorotaurine against Periodontal Pathogens. Int. J. Mol. Sci. 2024, 25, 8357.
https://doi.org/10.3390/ijms25158357
AMA Style
Kowalczyk K, Coraça-Huber DC, Wille-Kollmar W, Berktold M, Nagl M.
Activity of N-Chlorotaurine against Periodontal Pathogens. International Journal of Molecular Sciences. 2024; 25(15):8357.
https://doi.org/10.3390/ijms25158357
Chicago/Turabian Style
Kowalczyk, Kacper, Débora C. Coraça-Huber, Walter Wille-Kollmar, Michael Berktold, and Markus Nagl.
2024. "Activity of N-Chlorotaurine against Periodontal Pathogens" International Journal of Molecular Sciences 25, no. 15: 8357.
https://doi.org/10.3390/ijms25158357
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