SCD2 Regulation Targeted by miR-200c-3p on Lipogenesis Alleviates Mesenchymal Stromal Cell Senescence
Abstract
:1. Introduction
2. Results
2.1. MSCs at Late Passage Present Obvious Senescent Phenotypes
2.2. SCD2 Expression Is Enforced in Late Passage MSCs
2.3. SCD2 Replenishment Alleviates MSC Senescence by Boosting Lipogenesis
2.4. Prediction and Identification of Upstream miRNAs Targeting SCD2
2.5. MiR-200c-3p Over-Expression Accelerates MSC Senescence and Eliminates Lipogenesis
2.6. SCD2 Is Targeted by miR-200c-3p to Influence MSC Senescence via Regulating Lipogenesis
3. Discussion
4. Materials and Methods
4.1. MSC Isolation and Culture
4.2. Morphological Observation and Quantitative Analysis of MSCs
4.3. Senescence-Associated β-Galactosidase (SA-β-Gal) Staining
4.4. Construction of SCD2-Over-Expressed MSCs
4.5. Osteogenic Differentiation and Alizarin Red s (ARS) Staining
4.6. Nile Red Staining
4.7. Prediction for Upstream miRNAs of SCD2
4.8. Dual Luciferase Reporter Assay
4.9. Construction of miR-200c-3p-Over-Expressed MSCs
4.10. Gene Expression Analysis
4.11. Western Blot Analysis
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Yu, X.; Zhang, C.; Ma, Q.; Gao, X.; Sun, H.; Sun, Y.; Wang, Y.; Zhang, H.; Shi, Y.; Meng, X.; et al. SCD2 Regulation Targeted by miR-200c-3p on Lipogenesis Alleviates Mesenchymal Stromal Cell Senescence. Int. J. Mol. Sci. 2024, 25, 8538. https://doi.org/10.3390/ijms25158538
Yu X, Zhang C, Ma Q, Gao X, Sun H, Sun Y, Wang Y, Zhang H, Shi Y, Meng X, et al. SCD2 Regulation Targeted by miR-200c-3p on Lipogenesis Alleviates Mesenchymal Stromal Cell Senescence. International Journal of Molecular Sciences. 2024; 25(15):8538. https://doi.org/10.3390/ijms25158538
Chicago/Turabian StyleYu, Xiao, Chang Zhang, Qianhui Ma, Xingyu Gao, Hui Sun, Yanan Sun, Yuezeng Wang, Haiying Zhang, Yingai Shi, Xiaoting Meng, and et al. 2024. "SCD2 Regulation Targeted by miR-200c-3p on Lipogenesis Alleviates Mesenchymal Stromal Cell Senescence" International Journal of Molecular Sciences 25, no. 15: 8538. https://doi.org/10.3390/ijms25158538