Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein
Abstract
:1. Introduction
2. Results
3. Materials and Methods
3.1. Reagents
3.2. Gold-Coated Sensor Chip Preparation
3.3. Gold-Coated Sensor Chip Surface Stabilization
3.4. Nucleocapsid Protein Immobilization
3.5. Interaction of Immobilized SCoV2-rN with Specific Antibodies
3.6. Selection of Regeneration Solution and Regeneration Duration
3.7. Optimization of SCoV2-rN Surface Concentration
3.8. Calculations
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Antibody Type | Detection Method | Linear Range [ng/mL] | LOD [ng/mL] | Reference |
---|---|---|---|---|
n/a | electrochemical impedance transduction | − | 0.00195 (13 fM) | [14] |
Monoclonal humanized IgG | paper-based ELISA | 1–50 | 9 | [18] |
Rabbit polyclonal IgG | electrochemical immunosensor | 1 × 10−6–1 | 1 × 10−6 | [20] |
n/a | lateral flow immunoassay | − | 5 | [22] |
Polyclonal IgG | SPR spectroscopy | 100–10,000 | 45.6 | [25] |
Mouse monoclonal IgG | SPR spectroscopy | 75–7500 | 8.55 | This work |
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Lisyte, V.; Kausaite-Minkstimiene, A.; Brasiunas, B.; Popov, A.; Ramanaviciene, A. Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein. Int. J. Mol. Sci. 2024, 25, 8574. https://doi.org/10.3390/ijms25168574
Lisyte V, Kausaite-Minkstimiene A, Brasiunas B, Popov A, Ramanaviciene A. Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein. International Journal of Molecular Sciences. 2024; 25(16):8574. https://doi.org/10.3390/ijms25168574
Chicago/Turabian StyleLisyte, Viktorija, Asta Kausaite-Minkstimiene, Benediktas Brasiunas, Anton Popov, and Almira Ramanaviciene. 2024. "Surface Plasmon Resonance Immunosensor for Direct Detection of Antibodies against SARS-CoV-2 Nucleocapsid Protein" International Journal of Molecular Sciences 25, no. 16: 8574. https://doi.org/10.3390/ijms25168574