SOX2, JAGGED1, β-Catenin, and Vitamin D Receptor Expression Patterns during Early Development and Innervation of the Human Inner Ear
Abstract
:1. Introduction
2. Results
2.1. Morphology of the Developing Human Inner Ear
2.2. SOX2 and JAGGED1 Expression Characterizes Prosensory Domains of the Developing Human Inner Ear
2.3. Innervation of the Developing Human Inner Ear
2.4. CTNNB1 Expression in the Developing Human Inner Ear
2.5. VDR Expression in the Developing Human Inner Ear
3. Discussion
4. Materials and Methods
4.1. Human Samples
4.2. Immunofluorescence Staining
4.3. Innervation Analysis
4.4. Immunofluorescence Signal Quantification
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Antibodies | Host | Code No. | Dilution | Source | |
---|---|---|---|---|---|
Primary | Anti-Acetylated-alpha-tubulin | Mouse | 12152 | 1:500 | Cell Signaling Technology (CST), (Danvers, MA, USA) |
Anti-Beta-catenin | Mouse | 2677 | 1:200 | Cell Signaling Technology (CST), (Danvers, MA, USA) | |
Anti-Vitamin D Receptor | Mouse | sc-13133 | 1:50 | Santa Cruz Biotechnology, Dallas, TX, USA | |
Anti-JAGGED1 | Goat | AF1277 | 1:100 | R&D Systems, Minneapolis, MN, USA | |
Anti-SOX2 | Rabbit | 3579 | 1:400 | Cell Signaling Technology (CST), (Danvers, MA, USA) | |
Secondary | Alexa Fluor®488 Anti-Mouse lgG | Donkey | 715-545-150 | 1:400 | Jackson Immuno Research Laboratories, Inc., Baltimore, PA, USA |
Alexa Fluor®488 Anti-Rabbit lgG | Donkey | 711-545-152 | 1:400 | Jackson Immuno Research Laboratories, Inc., Baltimore, PA, USA | |
Rhodamine Red™-X Anti-Mouse IgG | Donkey | 715-295-151 | 1:400 | Jackson Immuno Research Laboratories, Inc., Baltimore, PA, USA | |
Rhodamine Red™-X Anti-Goat IgG | Donkey | 705-295-003 | 1:400 | Jackson Immuno Research Laboratories, Inc., Baltimore, PA, USA |
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Mikulić, P.; Ogorevc, M.; Petričević, M.; Kaličanin, D.; Tafra, R.; Saraga-Babić, M.; Mardešić, S. SOX2, JAGGED1, β-Catenin, and Vitamin D Receptor Expression Patterns during Early Development and Innervation of the Human Inner Ear. Int. J. Mol. Sci. 2024, 25, 8719. https://doi.org/10.3390/ijms25168719
Mikulić P, Ogorevc M, Petričević M, Kaličanin D, Tafra R, Saraga-Babić M, Mardešić S. SOX2, JAGGED1, β-Catenin, and Vitamin D Receptor Expression Patterns during Early Development and Innervation of the Human Inner Ear. International Journal of Molecular Sciences. 2024; 25(16):8719. https://doi.org/10.3390/ijms25168719
Chicago/Turabian StyleMikulić, Petra, Marin Ogorevc, Marin Petričević, Dean Kaličanin, Robert Tafra, Mirna Saraga-Babić, and Snježana Mardešić. 2024. "SOX2, JAGGED1, β-Catenin, and Vitamin D Receptor Expression Patterns during Early Development and Innervation of the Human Inner Ear" International Journal of Molecular Sciences 25, no. 16: 8719. https://doi.org/10.3390/ijms25168719