The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells
Abstract
:1. Introduction
2. Results
2.1. Quercetin Prevents Cellular Apoptosis and Defective Cell Functions in TM4 Cells Treated with FB1
2.2. Quercetin Eliminates Excess Oxidative Stress and Restores the Mitochondrial Membrane Potential Damage in TM4 Cells Treated with FB1
2.3. Dynamic Transcriptome Changes in TM4 Cells Treated with FB1 and Quercetin
2.4. Quercetin Treatment Restored the Defective Energy Metabolism Level in FB1-Treated TM4 Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatment
4.2. CCK-8 Assay
4.3. TUNEL Assay
4.4. Measurement of ROS, MDA and SOD Activity
4.5. RNA Extraction, cDNA Synthesis, and Real-Time PCR
4.6. Western Blot
4.7. MMP Detection
4.8. Measurement of ATP
4.9. Determination of Glucose, Pyruvate, and Lactic Acid Levels
4.10. Measurement of PKM and LDHA Activity
4.11. Mitochondrial Staining of Cultured Cells
4.12. RNA-Seq and Transcriptome Data Analysis
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ma, J.; Huang, R.; Zhang, H.; Liu, D.; Dong, X.; Xiong, Y.; Xiong, X.; Lan, D.; Fu, W.; He, H.; et al. The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells. Int. J. Mol. Sci. 2024, 25, 8764. https://doi.org/10.3390/ijms25168764
Ma J, Huang R, Zhang H, Liu D, Dong X, Xiong Y, Xiong X, Lan D, Fu W, He H, et al. The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells. International Journal of Molecular Sciences. 2024; 25(16):8764. https://doi.org/10.3390/ijms25168764
Chicago/Turabian StyleMa, Jun, Ruixue Huang, Huai Zhang, Dongju Liu, Xiaodong Dong, Yan Xiong, Xianrong Xiong, Daoliang Lan, Wei Fu, Honghong He, and et al. 2024. "The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells" International Journal of Molecular Sciences 25, no. 16: 8764. https://doi.org/10.3390/ijms25168764