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Article

Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults

by
Norma Zamora-Avilés
1,2,
Alonso A. Orozco-Flores
2,
Teodora Cavazos-Vallejo
2,
César I. Romo-Sáenz
2,
David A. Cuevas-García
2,
Ricardo Gomez-Flores
2 and
Patricia Tamez-Guerra
2,*
1
Departamento Ecología de Artrópodos y Manejo de Plagas, El Colegio de la Frontera Sur (ECOSUR), Carretera Antiguo Aeropuerto Km 2.5, Tapachula 30700, Mexico
2
Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(16), 8807; https://doi.org/10.3390/ijms25168807
Submission received: 3 July 2024 / Revised: 7 August 2024 / Accepted: 9 August 2024 / Published: 13 August 2024
(This article belongs to the Special Issue Advances in Genetics and Phylogenomics of Tree)
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Abstract

Beauveria bassiana has potential for Aedes aegypti biological control. However, its efficacy depends on the strain’s geographic location, host susceptibility, and virulence. The present study aimed to evaluate the effectiveness of B. bassiana strain BBPTG4 conidia in controlling Ae. aegypti adults and its detection via introns profile on exposed mosquito corpses. Morphologic characteristics among strains were highly similar. Comprehensive testing of these strains demonstrated that BBPT4 exhibited the ideal biological activity for Ae. aegypti control, with a median lethal time (TL50) of 7.5 d compared to ~3 d and ~10 d for BB01 and BB37 strains, respectively. Infected mosquitoes died after GHA and BBPTG4 exposure, and corpses were analyzed for infecting strains detection. Differences among the seven evaluated strains were determined, assessing five different insertion group I intron profiles in BBTG4, BB01, GHA, BB37, and BB02 strains. Mosquitoes infected by BBPTG4 and non-exposed (negative control) intron profiles were obtained. We detected the presence of introns in the BBPTG4 strain, which were not present in non-exposed mosquitoes. In conclusion, B. bassiana strains showed similarities in terms of their cultural and microscopic morphological characteristics and biologicals virulence level, but different intron profiles. BBPTG4 strain-infected Ae. aegypti adult corpses, showing specific amplicons, enabled us to identify B. bassiana at the strain level among infected mosquitoes. However, monitoring and detection of field-infected insects is essential for further verification.
Keywords: introns profile; dengue vector; entomopathogenic fungus; strain detection introns profile; dengue vector; entomopathogenic fungus; strain detection

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MDPI and ACS Style

Zamora-Avilés, N.; Orozco-Flores, A.A.; Cavazos-Vallejo, T.; Romo-Sáenz, C.I.; Cuevas-García, D.A.; Gomez-Flores, R.; Tamez-Guerra, P. Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults. Int. J. Mol. Sci. 2024, 25, 8807. https://doi.org/10.3390/ijms25168807

AMA Style

Zamora-Avilés N, Orozco-Flores AA, Cavazos-Vallejo T, Romo-Sáenz CI, Cuevas-García DA, Gomez-Flores R, Tamez-Guerra P. Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults. International Journal of Molecular Sciences. 2024; 25(16):8807. https://doi.org/10.3390/ijms25168807

Chicago/Turabian Style

Zamora-Avilés, Norma, Alonso A. Orozco-Flores, Teodora Cavazos-Vallejo, César I. Romo-Sáenz, David A. Cuevas-García, Ricardo Gomez-Flores, and Patricia Tamez-Guerra. 2024. "Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults" International Journal of Molecular Sciences 25, no. 16: 8807. https://doi.org/10.3390/ijms25168807

APA Style

Zamora-Avilés, N., Orozco-Flores, A. A., Cavazos-Vallejo, T., Romo-Sáenz, C. I., Cuevas-García, D. A., Gomez-Flores, R., & Tamez-Guerra, P. (2024). Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults. International Journal of Molecular Sciences, 25(16), 8807. https://doi.org/10.3390/ijms25168807

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