Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults
Abstract
:1. Introduction
2. Results
2.1. Macroscopic Morphological Description of Beauveria basssiana Strains
2.2. Virulence (LT50) of B. bassiana Strains against Ae. aegypti Adults
2.3. Subunit I Intron Amplification from DNA of B. bassiana Strains
2.4. BBPTG4 and GHA Introns Profile in Ae. aegypti Adults
3. Discussion
4. Materials and Methods
4.1. Mosquito Source and Rearing Conditions
4.2. B. bassiana Strains Source and Phenotypic Characterization
4.3. B. bassiana Strains Virulence against Ae. aegypti Adults
4.4. Molecular Characterization of Seven B. bassiana Strains
4.4.1. DNA Extraction
4.4.2. Profile of B. bassiana Strains by PCR
4.5. BBPTG4 Strain Detection in Ae. aegypti Adult Corpses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Strain | n a | Slope ±SE | Intercept ±SE | LT50 Fiducial Limits b (days) | χ2 c |
---|---|---|---|---|---|
BBPTG4 | 10 | 7.30 ± 0.88 | −6.39 ± 0.74 | 7.5 (6.96−8.04) | 8.33 |
GHA | 11 | 5.48 ± 0.52 | −3.93 ± 0.39 | 5.22 (4.80−5.66) | 2.53 |
BB42 | Nd | ||||
BB01 | 12 | 12.40 ± 1.70 | −12.21 ± 1.70 | 9.65 (9.24–10.15) | 7.60 |
BBPTG6 | 9 | 6.50 ± 0.84 | −4.34 ± 0.58 | 4.65 (4.24–5.07) | 5.25 |
BB37 | 8 | 5.27 ± 0.71 | −2.45 ± 0.39 | 2.92 (2.55–3.29) | 3.24 |
BB02 | 10 | 6.90 ± 0.92 | −4.63 ± 0.64 | 4.67 (4.25–5.13) | 1.97 |
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Zamora-Avilés, N.; Orozco-Flores, A.A.; Cavazos-Vallejo, T.; Romo-Sáenz, C.I.; Cuevas-García, D.A.; Gomez-Flores, R.; Tamez-Guerra, P. Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults. Int. J. Mol. Sci. 2024, 25, 8807. https://doi.org/10.3390/ijms25168807
Zamora-Avilés N, Orozco-Flores AA, Cavazos-Vallejo T, Romo-Sáenz CI, Cuevas-García DA, Gomez-Flores R, Tamez-Guerra P. Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults. International Journal of Molecular Sciences. 2024; 25(16):8807. https://doi.org/10.3390/ijms25168807
Chicago/Turabian StyleZamora-Avilés, Norma, Alonso A. Orozco-Flores, Teodora Cavazos-Vallejo, César I. Romo-Sáenz, David A. Cuevas-García, Ricardo Gomez-Flores, and Patricia Tamez-Guerra. 2024. "Intra-Phenotypic and -Genotypic Variations of Beauveria bassiana (Bals.) Vuill. Strains Infecting Aedes aegypti L. Adults" International Journal of Molecular Sciences 25, no. 16: 8807. https://doi.org/10.3390/ijms25168807