The Ubiquitin Ligase Adaptor NDFIP1 Interacts with TRESK and Negatively Regulates the Background K+ Current
Abstract
:1. Introduction
2. Results
2.1. The Coexpression of NDFIP1 with TRESK Reduces the Background K+ Current
2.2. The Effect of NDFIP1 on TRESK Is Mediated by E3 Ubiquitin Ligases
2.3. TRESK Regulation by NDFIP1 Does Not Depend on the Ubiquitination of a Single Lysine Residue in the Channel
2.4. TRESK Is Ubiquitinated When NDFIP1 Is Coexpressed
2.5. Protein–Protein Interaction between TRESK and NDFIP1
2.6. Coexpression of NDFIP1 Reduces the Quantity of TRESK Protein on the Oocyte Surface
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Molecular Biology
4.3. Two-Electrode Voltage Clamp
4.4. (Co-)Immunoprecipitation
4.5. Western Blot
4.6. Confocal Microscopy
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pergel, E.; Tóth, D.J.; Baukál, D.; Veres, I.; Czirják, G. The Ubiquitin Ligase Adaptor NDFIP1 Interacts with TRESK and Negatively Regulates the Background K+ Current. Int. J. Mol. Sci. 2024, 25, 8879. https://doi.org/10.3390/ijms25168879
Pergel E, Tóth DJ, Baukál D, Veres I, Czirják G. The Ubiquitin Ligase Adaptor NDFIP1 Interacts with TRESK and Negatively Regulates the Background K+ Current. International Journal of Molecular Sciences. 2024; 25(16):8879. https://doi.org/10.3390/ijms25168879
Chicago/Turabian StylePergel, Enikő, Dániel J. Tóth, Dóra Baukál, Irén Veres, and Gábor Czirják. 2024. "The Ubiquitin Ligase Adaptor NDFIP1 Interacts with TRESK and Negatively Regulates the Background K+ Current" International Journal of Molecular Sciences 25, no. 16: 8879. https://doi.org/10.3390/ijms25168879