Chronic Neuronal Hyperexcitation Exacerbates Tau Propagation in a Mouse Model of Tauopathy
Abstract
:1. Introduction
2. Results
2.1. Tau Pathology Propagation from EC to Hippocampus Following Tau Fibril Microinjection
2.2. Immunohistochemical Analysis of the Effects of Chronic Neuronal Stimulation on Tau Propagation
2.3. Biochemical Analysis of the Effects of Chronic Neuronal Stimulation on Tau Propagation
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Recombinant Tau Purification and Fibrilization
4.3. Stereotaxic Injection of AAV and Tau Fibrils
4.4. Optogenetics
4.5. Immunohistochemistry
4.6. Biochemistry
4.7. Immunoblots
4.8. Image Analysis
4.9. Human-Specific Tau ELISA
4.10. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nishida, I.; Yamada, K.; Sakamoto, A.; Wakabayashi, T.; Iwatsubo, T. Chronic Neuronal Hyperexcitation Exacerbates Tau Propagation in a Mouse Model of Tauopathy. Int. J. Mol. Sci. 2024, 25, 9004. https://doi.org/10.3390/ijms25169004
Nishida I, Yamada K, Sakamoto A, Wakabayashi T, Iwatsubo T. Chronic Neuronal Hyperexcitation Exacerbates Tau Propagation in a Mouse Model of Tauopathy. International Journal of Molecular Sciences. 2024; 25(16):9004. https://doi.org/10.3390/ijms25169004
Chicago/Turabian StyleNishida, Itaru, Kaoru Yamada, Asami Sakamoto, Tomoko Wakabayashi, and Takeshi Iwatsubo. 2024. "Chronic Neuronal Hyperexcitation Exacerbates Tau Propagation in a Mouse Model of Tauopathy" International Journal of Molecular Sciences 25, no. 16: 9004. https://doi.org/10.3390/ijms25169004