Quercetin Attenuates Acetaldehyde-Induced Cytotoxicity via the Heme Oxygenase-1-Dependent Antioxidant Mechanism in Hepatocytes
Abstract
:1. Introduction
2. Results
2.1. Cytoprotection against the Acetaldehyde Toxicity and ALDH Activity Enhancement by Quercetin
2.2. Inhibitory Effect of Quercetin on the Reactive Oxygen Species Accumulation Induced by Acetaldehyde
2.3. Up-Regulation of the Intracellular Antioxidant-Related Gene Expression by Quercetin
2.4. HO-1-Denpedent Inhibition of the Acetaldehyde-Induced ROS Elevation and Cytotoxicity by Quercetin
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Cultures and Viability Determination
4.3. Intracellular Reactive Oxygen Species Measurement and Image Analysis
4.4. RNA Extraction and Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
4.5. Glutathione Titration
4.6. ALDH Activity Assay
4.7. Western Blot Analysis
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Forward Primer | Reverse Primer | Cycles and Product Size |
---|---|---|---|
mβ-actin | 5′-GTCACCCACACTGTGCCCATCTA-3′ | 5′-GCAATGCCAGGGTACATGGTGGT-3′ | 16, 455 bp |
mALDH1A1 | 5′-GACAGGCTTTCCAGATTGGCTC-3′ | 5′-AAGACTTTCCCACCATTGAGTGC-3′ | 26, 142 bp |
mALDH2 | 5′-TGAAGACGGTTACTGTCAAAGTGC-3′ | 5′-AGTGTGTGTGGCGGTTTTTCTC-3′ | 26, 115 bp |
mALDH3 | 5′-GATGCCCATTGTGTGTGTTCG-3′ | 5′-CCACCGCTTGATGTCTCTGC-3′ | 26, 138 bp |
mHO-1 | 5′-ACATCGACAGCCCCACCAAGTTCAA-3′ | 5′-CTGACGAAGTGACGCCATCTGTGAG-3′ | 22, 203 bp |
mGCLC | 5′-GGCGATGTTCTTGAGACTCTGC-3′ | 5′-TTCCTTCGATCATGTAACTCCCATA-3′ | 26, 100 bp |
mxCT | 5′-CCTGGCATTTGGACGCTACAT-3′ | 5′-TGAGAATTGCTGTGAGCTTGCA-3′ | 25, 182 bp |
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Li, K.; Kidawara, M.; Chen, Q.; Munemasa, S.; Murata, Y.; Nakamura, T.; Nakamura, Y. Quercetin Attenuates Acetaldehyde-Induced Cytotoxicity via the Heme Oxygenase-1-Dependent Antioxidant Mechanism in Hepatocytes. Int. J. Mol. Sci. 2024, 25, 9038. https://doi.org/10.3390/ijms25169038
Li K, Kidawara M, Chen Q, Munemasa S, Murata Y, Nakamura T, Nakamura Y. Quercetin Attenuates Acetaldehyde-Induced Cytotoxicity via the Heme Oxygenase-1-Dependent Antioxidant Mechanism in Hepatocytes. International Journal of Molecular Sciences. 2024; 25(16):9038. https://doi.org/10.3390/ijms25169038
Chicago/Turabian StyleLi, Kexin, Minori Kidawara, Qiguang Chen, Shintaro Munemasa, Yoshiyuki Murata, Toshiyuki Nakamura, and Yoshimasa Nakamura. 2024. "Quercetin Attenuates Acetaldehyde-Induced Cytotoxicity via the Heme Oxygenase-1-Dependent Antioxidant Mechanism in Hepatocytes" International Journal of Molecular Sciences 25, no. 16: 9038. https://doi.org/10.3390/ijms25169038