Genome-Wide Identification of the DOF Gene Family in Kiwifruit (Actinidia chinensis) and Functional Validation of AcDOF22 in Response to Drought Stress
Abstract
:1. Introduction
2. Results
2.1. Identification, Characterization, and Phylogenetic Analysis of the DOF Genes in Kiwifruit
2.2. Evolutionary Analysis, Protein Motifs, Conserved Domains, and AcDOF Gene Structure
2.3. Analysis of cis-Regulatory Elements of AcDOFs
2.4. Collinearity and Synteny Analysis of AcDOFs
2.5. GO Annotation of the AcDOF Gene Family
2.6. Expression Patterns of AcDOFs in Kiwifruit
2.7. qRT-PCR Analysis of AcDOFs under Abiotic Stress
2.8. Subcellular Localization of AcDOF22
2.9. AcDOF22 Positively Regulates Kiwifruit Drought Resistance
2.10. AcDOF22 Transcriptionally Activates AcDERB2A Expression
3. Discussion
4. Materials and Methods
4.1. Identification of DOF Gene Family Members and Construction of a Phylogenetic Tree
4.2. Analysis of the Structural Domains and Conserved Motifs of the Kiwifruit DOF Gene Family
4.3. CREs Analysis
4.4. Chromosomal Distribution of AcDOF Genes, Duplication, and Synteny Analysis
4.5. GO-Based Annotation of AcDOFs
4.6. Retrieval of RNA-Seq Expression Profile Data
4.7. Kiwifruit Plant Treatment, RNA Extraction, and qRT-PCR Analysis
4.8. Subcellular Localization and Yeast Self-Activation
4.9. Agrobacterium-Mediated Transient Expression in Kiwifruit Leaves
4.10. Dual-Luciferase Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, C.; Bai, H.; Li, C.; Pang, Z.; Xuan, L.; Lv, D.; Niu, S. Genome-Wide Identification of the DOF Gene Family in Kiwifruit (Actinidia chinensis) and Functional Validation of AcDOF22 in Response to Drought Stress. Int. J. Mol. Sci. 2024, 25, 9103. https://doi.org/10.3390/ijms25169103
Zhao C, Bai H, Li C, Pang Z, Xuan L, Lv D, Niu S. Genome-Wide Identification of the DOF Gene Family in Kiwifruit (Actinidia chinensis) and Functional Validation of AcDOF22 in Response to Drought Stress. International Journal of Molecular Sciences. 2024; 25(16):9103. https://doi.org/10.3390/ijms25169103
Chicago/Turabian StyleZhao, Chao, Hao Bai, Chaoshuo Li, Zhaojin Pang, Lifeng Xuan, Dezhi Lv, and Shuaike Niu. 2024. "Genome-Wide Identification of the DOF Gene Family in Kiwifruit (Actinidia chinensis) and Functional Validation of AcDOF22 in Response to Drought Stress" International Journal of Molecular Sciences 25, no. 16: 9103. https://doi.org/10.3390/ijms25169103