Expression in Pichia pastoris of Thermostable Endo-1,4-β-xylanase from the Actinobacterium Nocardiopsis halotolerans: Properties and Use for Saccharification of Xylan-Containing Products
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characteristics of the Amino Acid Sequence and Phylogenetic Analysis
2.2. Cloning, Expression and Purification
2.3. Properties of NhX1
3. Materials and Methods
3.1. Microorganisms and Cultivation
3.2. Gene Cloning and Construction of a Recombinant Producer
3.3. Fermentation and Purification of NhX1
3.4. Characterization of NhX1
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lisov, A.V.; Belova, O.V.; Belov, A.A.; Lisova, Z.A.; Nagel, A.S.; Shadrin, A.M.; Andreeva-Kovalevskaya, Z.I.; Nagornykh, M.O.; Zakharova, M.V.; Leontievsky, A.A. Expression in Pichia pastoris of Thermostable Endo-1,4-β-xylanase from the Actinobacterium Nocardiopsis halotolerans: Properties and Use for Saccharification of Xylan-Containing Products. Int. J. Mol. Sci. 2024, 25, 9121. https://doi.org/10.3390/ijms25169121
Lisov AV, Belova OV, Belov AA, Lisova ZA, Nagel AS, Shadrin AM, Andreeva-Kovalevskaya ZI, Nagornykh MO, Zakharova MV, Leontievsky AA. Expression in Pichia pastoris of Thermostable Endo-1,4-β-xylanase from the Actinobacterium Nocardiopsis halotolerans: Properties and Use for Saccharification of Xylan-Containing Products. International Journal of Molecular Sciences. 2024; 25(16):9121. https://doi.org/10.3390/ijms25169121
Chicago/Turabian StyleLisov, Alexander V., Oksana V. Belova, Andrey A. Belov, Zoya A. Lisova, Alexey S. Nagel, Andrey M. Shadrin, Zhanna I. Andreeva-Kovalevskaya, Maxim O. Nagornykh, Marina V. Zakharova, and Alexey A. Leontievsky. 2024. "Expression in Pichia pastoris of Thermostable Endo-1,4-β-xylanase from the Actinobacterium Nocardiopsis halotolerans: Properties and Use for Saccharification of Xylan-Containing Products" International Journal of Molecular Sciences 25, no. 16: 9121. https://doi.org/10.3390/ijms25169121