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Open AccessArticle
Temporal and Spatial Variations in Zebrafish Hairy/E(spl) Gene Expression in Response to Mib1-Mediated Notch Signaling During Neurodevelopment
by
Yi-Chieh Chen
Yi-Chieh Chen 1,2,3,†
,
Fu-Yu Hsieh
Fu-Yu Hsieh 4,†,
Chia-Wei Chang
Chia-Wei Chang 4,
Mu-Qun Sun
Mu-Qun Sun 4 and
Yi-Chuan Cheng
Yi-Chuan Cheng 1,4,*
1
Neuroscience Research Center, Chang Gung Memorial Hospital, Linkou, Taoyuan 333423, Taiwan
2
Department of Neurology, Chang Gung Memorial Hospital at Linkou Medical Center, Taoyuan 333423, Taiwan
3
Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
4
Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(17), 9174; https://doi.org/10.3390/ijms25179174 (registering DOI)
Submission received: 24 July 2024
/
Revised: 17 August 2024
/
Accepted: 22 August 2024
/
Published: 23 August 2024
Abstract
Notch signaling is a conserved pathway crucial for nervous system development. Disruptions in this pathway are linked to neurodevelopmental disorders, neurodegenerative diseases, and brain tumors. Hairy/E(spl) (HES) genes, major downstream targets of Notch, are commonly used as markers for Notch activation. However, these genes can be activated, inhibited, or function independently of Notch signaling, and their response to Notch disruption varies across tissues and developmental stages. MIB1/Mib1 is an E3 ubiquitin ligase that enables Notch receptor activation by processing ligands like Delta and Serrate. We investigated Notch signaling disruption using the zebrafish Mib1 mutant line, mib1ta52b, focusing on changes in the expression of Hairy/E(spl) (her) genes. Our findings reveal significant variability in her gene expression across different neural cell types, regions, and developmental stages following Notch disruption. This variability questions the reliability of Hairy/E(spl) genes as universal markers for Notch activation, as their response is highly context-dependent. This study highlights the complex and context-specific nature of Notch signaling regulation. It underscores the need for a nuanced approach when using Hairy/E(spl) genes as markers for Notch activity. Additionally, it provides new insights into Mib1's role in Notch signaling, contributing to a better understanding of its involvement in Notch signaling-related disorders.
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MDPI and ACS Style
Chen, Y.-C.; Hsieh, F.-Y.; Chang, C.-W.; Sun, M.-Q.; Cheng, Y.-C.
Temporal and Spatial Variations in Zebrafish Hairy/E(spl) Gene Expression in Response to Mib1-Mediated Notch Signaling During Neurodevelopment. Int. J. Mol. Sci. 2024, 25, 9174.
https://doi.org/10.3390/ijms25179174
AMA Style
Chen Y-C, Hsieh F-Y, Chang C-W, Sun M-Q, Cheng Y-C.
Temporal and Spatial Variations in Zebrafish Hairy/E(spl) Gene Expression in Response to Mib1-Mediated Notch Signaling During Neurodevelopment. International Journal of Molecular Sciences. 2024; 25(17):9174.
https://doi.org/10.3390/ijms25179174
Chicago/Turabian Style
Chen, Yi-Chieh, Fu-Yu Hsieh, Chia-Wei Chang, Mu-Qun Sun, and Yi-Chuan Cheng.
2024. "Temporal and Spatial Variations in Zebrafish Hairy/E(spl) Gene Expression in Response to Mib1-Mediated Notch Signaling During Neurodevelopment" International Journal of Molecular Sciences 25, no. 17: 9174.
https://doi.org/10.3390/ijms25179174
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