Lipopolysaccharide Core Truncation in Invasive Escherichia coli O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain or Plasmid | Description | Source or Reference |
---|---|---|
43895 | E. coli O157:H7 ATCC 43895, a clinical isolate, stx1+/stx2+, curli-positive | ATCC * |
manC-Tn5 | Tn5 inserted in manC of 43895 | [13] |
per-Tn5 | Tn5 inserted in per of 43895 | [13] |
wzy-Tn5 | Tn5 inserted into wzy of 43895 | [13] |
∆waaI | 43895 with waaI deletion | This work |
∆waaG | 43895 with waaG deletion | This work |
∆waaF | 43895 with waaF deletion | This work |
∆waaC | 43895 with waaC deletion | This work |
∆fliC | 43895 with fliC deletion | This work |
pKD3 | Template plasmid for mutagenesis (AmpR,CmR) | [46] |
pKD46 | Red recombinase helper plasmid, RepA101(Ts), AmpR | [46] |
pCP20 | Temperature-sensitive replicon, thermal induction of FLP synthesis, AmpR,CmR | [46] |
Primer Name | Sequence (5′-3′) |
---|---|
waaI-F | ttagaagcatttttctttataatactttaaataattaaaaatGTGTAGGCTGGAGCTGCTTCG |
waaI-R | atgtctcaactcaatgatagtgacatcatcctttttgagtataCATATGAATATCCTCCTTA |
waaG-F | tcaaccatccagaccacccgttatgatatccgccgctttctctGTGTAGGCTGGAGCTGCTTCG |
waaG-R | atgatcgttgctttttgtttatataaatattttccctttggcggtCATATGAATATCCTCCTTA |
waaF-F | atgaaaatactggtgatcggcccgtcttgggttggcgacatgaGTGTAGGCTGGAGCTGCTTCG |
waaF-R | tcaggcttcctcttgtaacaatagcgcgttgagttctttcagtaCATATGAATATCCTCCTTA |
waaC-F | atgcgggttttgatcgttaaaacatcgtcgatgggcgatgttctGTGTAGGCTGGAGCTGCTTCG |
waaC-R | tcatcttatctccgatgtcaacttattggttattatttcaagaCATATGAATATCCTCCTTA |
fliC-F | ttaaccctgcagcagagacagaacctgctgcggtacctggttaGTGTAGGCTGGAGCTGCTTCG |
fliC-R | atggcacaagtcattaataccaacagcctctcgctgatcactcaCATATGAATATCCTCCTTA |
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Sheng, H.; Ndeddy Aka, R.J.; Wu, S. Lipopolysaccharide Core Truncation in Invasive Escherichia coli O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability. Int. J. Mol. Sci. 2024, 25, 9224. https://doi.org/10.3390/ijms25179224
Sheng H, Ndeddy Aka RJ, Wu S. Lipopolysaccharide Core Truncation in Invasive Escherichia coli O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability. International Journal of Molecular Sciences. 2024; 25(17):9224. https://doi.org/10.3390/ijms25179224
Chicago/Turabian StyleSheng, Haiqing, Robinson J. Ndeddy Aka, and Sarah Wu. 2024. "Lipopolysaccharide Core Truncation in Invasive Escherichia coli O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability" International Journal of Molecular Sciences 25, no. 17: 9224. https://doi.org/10.3390/ijms25179224