Genome-Wide Analysis and Expression Profiling of Soybean RbcS Family in Response to Plant Hormones and Functional Identification of GmRbcS8 in Soybean Mosaic Virus
Abstract
:1. Introduction
2. Results
2.1. Identification and Physicochemical Characterization of GmRbcS Family Members
2.2. Conserved Motif, Gene Structure, and Domain Analysis
2.3. Phylogenetic Construction, Chromosome Distribution, and Collinearity Analysis
2.4. Comparative Comprehensive Map of RbcSs in Different Species
2.5. Cis-Acting Elements Analysis of GmRbcSs Promoter
2.6. Protein–Protein Interaction (PPI) Network in Soybean
2.7. Quantitative Analysis of GmRbcSs Genes in Different Tissues
2.8. Expression Patterns of GmRbcSs under Hormone Stresses
2.9. Transient Silencing GmRbcS8 Increases SMV Accumulation in Soybean
3. Discussion
4. Materials and Methods
4.1. Characterization and Physicochemical Properties of the RbcS Family in Soybean
4.2. Phylogenetic and Structure Analysis
4.3. Chromosomal Mapping and Gene Replication
4.4. Gene Regulatory Network Analysis
4.5. Analysis of Cis-Acting Elements in Promoter Sequences
4.6. Spatiotemporal Expression Analysis
4.7. Soybean Materials and Hormone Treatments
4.8. Total RNA Extraction and RT-qPCR Analysis
4.9. Virus-Induced Gene Silencing System (VIGS)
4.10. Assessing the Response of Silenced GmRbcS8 Gene to SMV
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene ID | Gene Name | Protein Length (aa) | Molecular Weight (kDa) | Theoretical Isoelectric Point (pI) | Instability Index | GRAVY | Subcellular Location |
---|---|---|---|---|---|---|---|
Glyma.08G365300 | GmRbcS1 | 90 | 9.97 | 8.89 | 43.52 | −0.12 | Chloroplast |
Glyma.13G046200 | GmRbcS2 | 178 | 20.02 | 8.87 | 30.71 | −0.24 | Chloroplast |
Glyma.13G097100 | GmRbcS3 | 172 | 19.60 | 8.95 | 53.39 | −0.35 | Chloroplast |
Glyma.14G089500 | GmRbcS4 | 159 | 17.83 | 9.81 | 45.98 | −0.53 | Chloroplast |
Glyma.18G182200 | GmRbcS5 | 100 | 11.54 | 9.17 | 49.22 | −0.33 | Chloroplast |
Glyma.18G182300 | GmRbcS6 | 131 | 15.09 | 9.20 | 50.32 | −0.48 | Chloroplast |
Glyma.18G296900 | GmRbcS7 | 150 | 16.76 | 9.22 | 43.77 | −0.14 | Chloroplast |
Glyma.19G046600 | GmRbcS8 | 178 | 20.01 | 8.87 | 31.32 | −0.24 | Chloroplast |
Glyma.19G046800 | GmRbcS9 | 178 | 20.01 | 8.87 | 31.32 | −0.24 | Chloroplast |
Glyma.19G046900 | GmRbcS10 | 135 | 14.81 | 9.39 | 33.23 | −0.06 | Chloroplast |
Glyma.19G047000 | GmRbcS11 | 105 | 12.41 | 7.81 | 36.75 | −0.45 | Chloroplast |
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Zhou, F.; Feng, W.; Mou, K.; Yu, Z.; Zeng, Y.; Zhang, W.; Zhou, Y.; Li, Y.; Gao, H.; Xu, K.; et al. Genome-Wide Analysis and Expression Profiling of Soybean RbcS Family in Response to Plant Hormones and Functional Identification of GmRbcS8 in Soybean Mosaic Virus. Int. J. Mol. Sci. 2024, 25, 9231. https://doi.org/10.3390/ijms25179231
Zhou F, Feng W, Mou K, Yu Z, Zeng Y, Zhang W, Zhou Y, Li Y, Gao H, Xu K, et al. Genome-Wide Analysis and Expression Profiling of Soybean RbcS Family in Response to Plant Hormones and Functional Identification of GmRbcS8 in Soybean Mosaic Virus. International Journal of Molecular Sciences. 2024; 25(17):9231. https://doi.org/10.3390/ijms25179231
Chicago/Turabian StyleZhou, Fangxue, Wenmi Feng, Kexin Mou, Zhe Yu, Yicheng Zeng, Wenping Zhang, Yonggang Zhou, Yaxin Li, Hongtao Gao, Keheng Xu, and et al. 2024. "Genome-Wide Analysis and Expression Profiling of Soybean RbcS Family in Response to Plant Hormones and Functional Identification of GmRbcS8 in Soybean Mosaic Virus" International Journal of Molecular Sciences 25, no. 17: 9231. https://doi.org/10.3390/ijms25179231