Loss of Lipooligosaccharide Synthesis in Acinetobacter baumannii Produces Changes in Outer Membrane Vesicle Protein Content
Abstract
:1. Introduction
2. Results
2.1. Comparison of Methods for Purifying A. baumannii OMVs
2.2. Comparison of ATCC 19606 and IB010 OMVs
2.3. Proteomic Characterization of ATCC 19606 and IB010 OMVs
2.4. Computational Characterization of Differentially Expressed Proteins
3. Discussion
4. Materials and Methods
4.1. Purification of A. baumannii OMVs
4.2. Nanoparticle Tracking Analysis (NTA)
4.3. Electron Microscopy and Cryoelectron Microscopy
4.4. Mass Spectrometry Analysis (LC-MS/MS)
4.5. Subcellular Localization and Epitope Prediction
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Protein ID | Gene Name | Protein Description | Combined Total Peptides |
---|---|---|---|
D0CG85 | tuf | Elongation factor Tu (Fragment) | 5 |
D0CDQ1 | bpeB | Efflux pump membrane transporter | 4 |
D0C629 | HMPREF0010_00209 | Translocation and assembly module subunit TamA | 3 |
D0CEK8 | atpF | ATP synthase subunit b | 3 |
D0C807 | gltI | Glutamate-aspartate periplasmic-binding protein | 2 |
D0CCD3 | HMPREF0010_02413 | Uncharacterized protein | 6 |
D0C7Q4 | HMPREF0010_00784 | Uncharacterized protein | 2 |
Protein ID | Gene Name | Protein Description | Combined Total Peptides |
---|---|---|---|
D0C7A8 | HMPREF0010_00638 | Lipoprotein | 3 |
D0CDQ8 | lolA | Outer-membrane lipoprotein carrier protein | 3 |
D0C9M0 | lolB | Outer-membrane lipoprotein LolB | 2 |
D0CC74 | rpsB | 30S ribosomal protein S2 | 2 |
D0CDB4 | HMPREF0010_02744 | DUF306 domain-containing protein | 2 |
D0CDY8 | HMPREF0010_02968 | TonB-dependent siderophore receptor | 2 |
D0CF71 | HMPREF0010_03537 | Carbapenem susceptibility porin CarO | 2 |
D0CFX6 | HMPREF0010_03656 | Metallo-beta-lactamase domain protein | 2 |
D0CBN6 | HMPREF0010_02166 | Uncharacterized protein | 3 |
D0CF26 | HMPREF0010_03356 | Uncharacterized protein | 3 |
D0C7G2 | HMPREF0010_00692 | Uncharacterized protein | 2 |
D0CB14 | HMPREF0010_01944 | Uncharacterized protein | 2 |
Protein ID | Gene Name | Protein Description | IB010 vs. ATCC 19606 log2 Fold Change | p Value |
---|---|---|---|---|
| ||||
D0C9R5 | HMPREF0010_01378 | OmpA family protein | 4.19 | <0.01 |
D0C907 | HMPREF0010_01565 | META domain protein (Fragment) | 2.81 | <0.01 |
D0CEN2 | HMPREF0010_03212 | Lipoprotein | 2.8 | <0.01 |
D0CCX7 | ttg2D | Toluene tolerance protein Ttg2D | 2.46 | <0.01 |
D0C6H4 | HMPREF0010_00354 | Outer membrane protein | 2.19 | <0.01 |
D0C5N9 | HMPREF0010_00069 | Signal peptide protein | 2.15 | <0.01 |
D0CDW1 | HMPREF0010_02941 | Lipoprotein | 2.15 | <0.01 |
D0CDU8 | HMPREF0010_02928 | Lipoprotein | 2.1 | 0.02 |
D0C6J4 | gdhB | Quinoprotein glucose dehydrogenase B | 2.06 | <0.01 |
D0CBP8 | mucD | Periplasmic serine endoprotease DegP-like | 1.84 | <0.01 |
D0C608 | HMPREF0010_00188 | DJ-1/PfpI family protein | 1.83 | <0.01 |
D0C8L9 | HMPREF0010_01099 | Hemolysin | 1.79 | 0.01 |
D0CDA9 | HMPREF0010_02739 | Uncharacterized protein | 5.62 | <0.01 |
D0CBW9 | HMPREF0010_02249 | Uncharacterized protein | 3.44 | <0.01 |
D0CFX4 | HMPREF0010_03654 | Uncharacterized protein | 2.86 | <0.01 |
D0C5H7 | HMPREF0010_00007 | Uncharacterized protein | 2.05 | 0.01 |
| ||||
D0CEW0 | ptk | Tyrosine-protein kinase Ptk (Fragment) | −4.48 | <0.01 |
D0CBK4 | HMPREF0010_02134 | Rhombotarget A | −3.99 | <0.01 |
D0C5L7 | mrcB | Penicillin-binding protein 1B | −3.78 | <0.01 |
D0CBK5 | HMPREF0010_02135 | Gammaproteobacterial enzyme transmembrane domain protein | −3.36 | 0.01 |
D0CD03 | rpsC | 30S ribosomal protein S3 | −3.22 | <0.01 |
D0C9Z6 | mqo | Probable malate:quinone oxidoreductase | −3.15 | <0.01 |
D0C8P3 | HMPREF0010_01123 | Type VI secretion system effector, Hcp1 family | −2.93 | 0.01 |
D0CDE5 | gcd | Quinoprotein glucose dehydrogenase | −2.45 | <0.01 |
D0CEV8 | HMPREF0010_03288 | Polysaccharide biosynthesis/export protein | −2.06 | <0.01 |
D0CC83 | mltB | Lytic murein transglycosylase B | −1.67 | <0.01 |
D0CBZ2 | HMPREF0010_02272 | Peptidase, M23 family | −1.62 | <0.01 |
D0CDN5 | HMPREF0010_02865 | Tat pathway signal sequence domain protein | −1.53 | <0.01 |
D0C6H8 | HMPREF0010_00358 | Uncharacterized protein | −3.66 | <0.01 |
D0C6N7 | HMPREF0010_00417 | Uncharacterized protein | −3.44 | <0.01 |
D0C985 | HMPREF0010_01198 | Uncharacterized protein | −2.75 | <0.01 |
D0CD52 | HMPREF0010_02682 | Uncharacterized protein | −2.5 | <0.01 |
D0C5L6 | HMPREF0010_00046 | Uncharacterized protein | −1.95 | <0.01 |
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Cano-Castaño, B.; Corral-Lugo, A.; Gato, E.; Terrón, M.C.; Martín-Galiano, A.J.; Sotillo, J.; Pérez, A.; McConnell, M.J. Loss of Lipooligosaccharide Synthesis in Acinetobacter baumannii Produces Changes in Outer Membrane Vesicle Protein Content. Int. J. Mol. Sci. 2024, 25, 9272. https://doi.org/10.3390/ijms25179272
Cano-Castaño B, Corral-Lugo A, Gato E, Terrón MC, Martín-Galiano AJ, Sotillo J, Pérez A, McConnell MJ. Loss of Lipooligosaccharide Synthesis in Acinetobacter baumannii Produces Changes in Outer Membrane Vesicle Protein Content. International Journal of Molecular Sciences. 2024; 25(17):9272. https://doi.org/10.3390/ijms25179272
Chicago/Turabian StyleCano-Castaño, Beatriz, Andrés Corral-Lugo, Eva Gato, María C. Terrón, Antonio J. Martín-Galiano, Javier Sotillo, Astrid Pérez, and Michael J. McConnell. 2024. "Loss of Lipooligosaccharide Synthesis in Acinetobacter baumannii Produces Changes in Outer Membrane Vesicle Protein Content" International Journal of Molecular Sciences 25, no. 17: 9272. https://doi.org/10.3390/ijms25179272