Commonalities and Specificities in Wheat (Triticum aestivum L.) Responses to Aluminum Toxicity and Low Phosphorus Revealed by Transcriptomics and Targeted Metabolomics
Abstract
:1. Introduction
2. Results
2.1. Wheat Root Transcriptome Profiling in Response to Al and LP Stresses
2.2. Identified Differentially Expressed Gene Responses to Al and LP Stresses
2.3. GO Annotations and KEGG Enrichment Analyses of DEGs under Al and LP Stresses
2.4. DEGs Related to Transcription Factors
2.5. Confirmation of RNA-Seq Data Using qRT-PCR
2.6. Joint Analysis of Genes and Metabolites Involved in Flavonoid Metabolism
2.7. Joint Analysis of Carbohydrate Metabolism Genes and Metabolites
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatment
4.2. RNA Extraction, Library Preparation, Sequencing, and Read Mapping
4.3. Differential Expression and Functional Enrichment Analysis
4.4. Quantitative Real-Time PCR (qRT-PCR) Validation
4.5. Targeted Metabolite Extraction and Profiling
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviation
EL | electrolyte leakage |
MDA | malondialdehyde |
OA | organic acid |
O2− | superoxide anion |
POD | peroxidase |
ROS | reactive oxygen species |
SOD | superoxide dismutase |
TF | transcription factor |
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Luo, D.; Li, Q.; Pang, F.; Zhang, W.; Li, Y.; Xing, Y.; Dong, D. Commonalities and Specificities in Wheat (Triticum aestivum L.) Responses to Aluminum Toxicity and Low Phosphorus Revealed by Transcriptomics and Targeted Metabolomics. Int. J. Mol. Sci. 2024, 25, 9273. https://doi.org/10.3390/ijms25179273
Luo D, Li Q, Pang F, Zhang W, Li Y, Xing Y, Dong D. Commonalities and Specificities in Wheat (Triticum aestivum L.) Responses to Aluminum Toxicity and Low Phosphorus Revealed by Transcriptomics and Targeted Metabolomics. International Journal of Molecular Sciences. 2024; 25(17):9273. https://doi.org/10.3390/ijms25179273
Chicago/Turabian StyleLuo, Daozhen, Qing Li, Fei Pang, Wenjie Zhang, Yangrui Li, Yongxiu Xing, and Dengfeng Dong. 2024. "Commonalities and Specificities in Wheat (Triticum aestivum L.) Responses to Aluminum Toxicity and Low Phosphorus Revealed by Transcriptomics and Targeted Metabolomics" International Journal of Molecular Sciences 25, no. 17: 9273. https://doi.org/10.3390/ijms25179273