ZIF-8 as a pH-Responsive Nanoplatform for 5-Fluorouracil Delivery in the Chemotherapy of Oral Squamous Cell Carcinoma
Abstract
:1. Introduction
2. Results
2.1. Loading and Release of 5-FU@ZIF-8
2.2. In Vitro Cell Compatibility against SCC7 Cell Line
2.3. RNA Sequencing Analysis Revealed Molecular Targets of the DDS
3. Discussion
3.1. The Trial and Error behind the Loading and Release of 5-FU@ZIF-8
3.2. In Vitro Cell Culture Studies and RNA Sequencing Analysis
4. Materials and Methods
4.1. Materials
4.2. Creation of a 5-FU Standard Curve
4.3. Synthesis of 5-FU-Loaded ZIF-8
4.4. In Vitro Quantification of 5-FU Release
4.5. Cell Culturing
4.6. In Vitro Cell Compatibility Studies
4.7. RNA Sequencing Methods
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variables of Absorption | Loaded Drug Capacity (mg/g) | |
---|---|---|
Type of heat-activated carrier | Clinoptilolite | No change |
ZIF-8 | 271.41 ± 21.94 | |
ZIF-90 | No change | |
Heat activation of ZIF-8 | 160 °C for 24 h | 271.41 ± 21.94 |
None | No change | |
5-FU/ZIF-8 ratio | 1/3 | 101.42 ± 10.24 |
1/2 | 150.99 ± 2.11 | |
1/1 | 271.41 ± 19.07 | |
4/1 | 248.38 ± 9.84 | |
Solvent | dH2O | 200.00 ± 1.95 |
PBS | 179.68 ± 21.73 | |
Methanol | No change |
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Hao, J.; Chen, C.; Pavelic, K.; Ozer, F. ZIF-8 as a pH-Responsive Nanoplatform for 5-Fluorouracil Delivery in the Chemotherapy of Oral Squamous Cell Carcinoma. Int. J. Mol. Sci. 2024, 25, 9292. https://doi.org/10.3390/ijms25179292
Hao J, Chen C, Pavelic K, Ozer F. ZIF-8 as a pH-Responsive Nanoplatform for 5-Fluorouracil Delivery in the Chemotherapy of Oral Squamous Cell Carcinoma. International Journal of Molecular Sciences. 2024; 25(17):9292. https://doi.org/10.3390/ijms25179292
Chicago/Turabian StyleHao, Jessica, Chider Chen, Kresimir Pavelic, and Fusun Ozer. 2024. "ZIF-8 as a pH-Responsive Nanoplatform for 5-Fluorouracil Delivery in the Chemotherapy of Oral Squamous Cell Carcinoma" International Journal of Molecular Sciences 25, no. 17: 9292. https://doi.org/10.3390/ijms25179292