Platelet-Rich Plasma (PRP) Based on Simple and Efficient Integrated Preparation Precises Quantitatively for Skin Wound Repair
Abstract
:1. Introduction
2. Results
2.1. Effect of RCF and Blood Storage Time on Platelet Recovery Rate
2.2. Optimizing the Platelet Recovery Rate of PRP Using Response Surface Methodology
2.2.1. Model Fitting and Variance Analysis
2.2.2. Effect of Variables on Platelet Recovery Rate
2.2.3. Model Verification
2.2.4. PRP Stability Analysis
2.3. Growth Factor Analysis for PRP and PPP
2.4. In Vitro Cellular Effects of PPP and PRP with Different Platelet Concentrations
2.5. In Vivo Evaluation of Wound Healing Efficacy
2.5.1. Wound Appearance Observation and Evaluation
2.5.2. H&E and Masson Staining
2.5.3. CD31 Staining
2.5.4. Collagen I/Collagen III Staining
2.5.5. iNOS Staining
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Materials
4.3. Factors Affecting the PRP Preparation Process
4.3.1. Effect of Relative Centrifugal Force (RCF) on Platelet Recovery Rate
4.3.2. Effect of Blood Storage Time on Platelet Recovery Rate
4.4. Optimizing Platelet Recovery Rate of PRP Using Response Surface Methodology
PRP Stability Analysis Method
4.5. PRP and PPP Activation and Growth Factor Quantification
4.6. In Vitro Activity Studies of PPP and PRP with Different Platelet Concentrations
- (1)
- PRP (8×) has an enrichment ratio of 8 to 9 times,
- (2)
- PRP (2×) has an enrichment ratio of 2 to 3 times,
- (3)
- PPP group,
- (4)
- Control group.
4.6.1. Cell Proliferation Assay
4.6.2. Cell Growth Assay
4.6.3. Cell Migration Assay
4.6.4. In Vitro Angiogenesis
4.7. In Vivo Activity Studies of PPP and PRP with Different Platelet Concentrations
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
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Qiu, M.; He, Y.; Zhang, H.; Zheng, Y.; Shi, X.; Yang, J. Platelet-Rich Plasma (PRP) Based on Simple and Efficient Integrated Preparation Precises Quantitatively for Skin Wound Repair. Int. J. Mol. Sci. 2024, 25, 9340. https://doi.org/10.3390/ijms25179340
Qiu M, He Y, Zhang H, Zheng Y, Shi X, Yang J. Platelet-Rich Plasma (PRP) Based on Simple and Efficient Integrated Preparation Precises Quantitatively for Skin Wound Repair. International Journal of Molecular Sciences. 2024; 25(17):9340. https://doi.org/10.3390/ijms25179340
Chicago/Turabian StyleQiu, Mengjie, Yating He, Haijie Zhang, Yunquan Zheng, Xianai Shi, and Jianmin Yang. 2024. "Platelet-Rich Plasma (PRP) Based on Simple and Efficient Integrated Preparation Precises Quantitatively for Skin Wound Repair" International Journal of Molecular Sciences 25, no. 17: 9340. https://doi.org/10.3390/ijms25179340