Populus trichocarpa EXPA6 Facilitates Radial and Longitudinal Transport of Na+ under Salt Stress
Abstract
:1. Introduction
2. Results
2.1. Expression of PtEXPA6 in Root and Shoot of Populus trichocarpa
2.2. Homologous Sequence Analysis of PtEXPA6
2.3. Overexpression of PtEXPA6 in Poplars
2.4. Effect of Salinity on Shoot Growth of PtEXPA6-Overexpressed Poplars
2.5. Effect of Salinity on Photosynthesis of PtEXPA6-Overexpressing Poplars
2.6. The Activity and Transcription of Antioxidant Enzymes
2.7. Na+ Content in Roots, Stems, and Leaves of Transgenic Poplars
2.8. Comparative Contractability and Comparative Extensibility of Root Cell Walls
2.9. Na+ Flux of Root Xylem and the Response to Osmotic Jump
2.10. Na+ Flux of Leaf Petiole Vascular Bundles and the Response to Osmotic Jump
2.11. The Correlation Analysis and Principal Component Analysis (PCA)
3. Discussion
3.1. PtEXPA6 Negatively Regulates Salt Tolerance in Transgenic Poplars
3.2. PtEXPA6 Increases Na+ Transport from Root to Shoot under Salt Stress
3.3. PtEXPA6 Influences Photosynthesis under Salt Stress
3.4. PtEXPA6 Influences ROS Scavenging Capacity under Salt Stress
4. Materials and Methods
4.1. Total RNA Isolation, PtEXPA6 Cloning, and Sequence Analysis
4.2. PtEXPA6 Overexpression in Poplars
4.3. Western Blotting
4.4. Comparative Extensibility and Contractility of Root Tips
4.5. Phenotype Test of Salt Tolerance
4.5.1. Measurement of Growth
4.5.2. Measurement of the Relative Electrolyte Leakage (REL)
4.5.3. Measurement of Leaf Gas Exchange and Chlorophyll Fluorescence
4.6. Determination of Antioxidant Enzyme Activity
4.7. Na+ Concentration in the Roots, Leaves and Stems
4.8. Flux Records of Na+ in the Root Xylem and Leaf Petiole Vascular Bundle
4.9. RT-qPCR Analysis
4.10. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, Z.; Yin, K.; Zhang, Y.; Yan, C.; Zhao, Z.; Li, J.; Liu, Y.; Feng, B.; Zhao, R.; Liu, J.; et al. Populus trichocarpa EXPA6 Facilitates Radial and Longitudinal Transport of Na+ under Salt Stress. Int. J. Mol. Sci. 2024, 25, 9354. https://doi.org/10.3390/ijms25179354
Liu Z, Yin K, Zhang Y, Yan C, Zhao Z, Li J, Liu Y, Feng B, Zhao R, Liu J, et al. Populus trichocarpa EXPA6 Facilitates Radial and Longitudinal Transport of Na+ under Salt Stress. International Journal of Molecular Sciences. 2024; 25(17):9354. https://doi.org/10.3390/ijms25179354
Chicago/Turabian StyleLiu, Zhe, Kexin Yin, Ying Zhang, Caixia Yan, Ziyan Zhao, Jing Li, Yi Liu, Bing Feng, Rui Zhao, Jian Liu, and et al. 2024. "Populus trichocarpa EXPA6 Facilitates Radial and Longitudinal Transport of Na+ under Salt Stress" International Journal of Molecular Sciences 25, no. 17: 9354. https://doi.org/10.3390/ijms25179354