Transcriptomic Insights into Salt Stress Response in Two Pepper Species: The Role of MAPK and Plant Hormone Signaling Pathways
Abstract
:1. Introduction
2. Results
2.1. Physiological and Phenotypical Alterations
2.2. Stress Tolerance Index Percentage (STI%)
2.3. Microscopic Scanning of Leaf and Root Tissues
2.4. Analysis of RNA Sequencing
2.5. Identification of the Module Using WGCNA
2.6. GO and KEGG Enrichment Analysis
2.7. MAPK Signaling Pathway
2.8. Plant Hormone Signal Transduction Pathway
2.9. qRT-PCR Validation
3. Discussion
3.1. Phenotypical and Physiological Changes
3.2. Role of MAPK Signaling Pathway
3.3. Role Hormone Signal Transduction Pathway
4. Materials and Methods
4.1. Plant Material and NaCl Treatments
4.2. Response of Biochemical Indexes
4.3. Stress Tolerance Index Percentage (STI%)
4.4. Scanning of Leaf and Root
4.5. RNA Extraction, Library Construction, and RNA Sequencing
4.6. Co-Expression Modules Identification
4.7. qRT-PCR Validation
4.8. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Farooq, M.A.; Zeeshan Ul Haq, M.; Zhang, L.; Wu, S.; Mushtaq, N.; Tahir, H.; Wang, Z. Transcriptomic Insights into Salt Stress Response in Two Pepper Species: The Role of MAPK and Plant Hormone Signaling Pathways. Int. J. Mol. Sci. 2024, 25, 9355. https://doi.org/10.3390/ijms25179355
Farooq MA, Zeeshan Ul Haq M, Zhang L, Wu S, Mushtaq N, Tahir H, Wang Z. Transcriptomic Insights into Salt Stress Response in Two Pepper Species: The Role of MAPK and Plant Hormone Signaling Pathways. International Journal of Molecular Sciences. 2024; 25(17):9355. https://doi.org/10.3390/ijms25179355
Chicago/Turabian StyleFarooq, Muhammad Aamir, Muhammad Zeeshan Ul Haq, Liping Zhang, Shuhua Wu, Naveed Mushtaq, Hassam Tahir, and Zhiwei Wang. 2024. "Transcriptomic Insights into Salt Stress Response in Two Pepper Species: The Role of MAPK and Plant Hormone Signaling Pathways" International Journal of Molecular Sciences 25, no. 17: 9355. https://doi.org/10.3390/ijms25179355