Specific microRNA Profile Associated with Inflammation and Lipid Metabolism for Stratifying Allergic Asthma Severity
Abstract
:1. Introduction
2. Results
2.1. Subject Classification in Study Cohort
2.2. Severe Uncontrolled Allergic Asthmatic Patients Display a Unique miRNA Fingerprint in Serum
2.3. Inflammation, Angiogenesis and Lipid Metabolism Signatures Are Enriched in Severe Allergic Asthmatic Patients
2.4. 4 Severe Allergic Asthma miRNA Profile Correlates with Dysregulated Lipid Metabolism
2.5. DE miRNAs Can Accurately Classify Allergic Asthmatic Patients According to Their Severity
2.6. Validation of miRNAs as Biomarkers for Severity Degree in Allergic Asthma
3. Discussion
4. Materials and Methods
4.1. Patients
4.2. miRNAs Extraction and Expression Quantification
4.3. Statistics
4.4. Functional Enrichment Analysis
4.5. Random Forest-Based Classifier
4.6. miRNA Validation Analysis in CAMP (Childhood Asthma Management Program) Cohort
4.7. Data Analysis of miRNA Validation in CAMP Cohort
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Control | Mild | Severe Uncontrolled | |
---|---|---|---|
n | 15 | 11 | 10 |
Age (years) | 37.4 ± 13.95 * | 36 ± 6.53 * | 50.9 ± 10.62 |
Onset (years) | - | 14.55 ± 10.21 | 10.7 ± 6.11 |
Gender (%F/%M) | 60/40 | 63.64/36.36 | 60/40 |
BMI | 28.71 ± 5.65 | 27.52 ± 5.80 | 28.35 ± 4.00 |
Smoker (%) | 20 | 9.09 | 0 |
Ex-smoker (%) | 0 | 0 | 20 |
Total IgE (U) | - | 551.09 ± 733.8 | 462.1 ± 587.92 |
AC (%) | 0 | 0 * | 60 |
AH (%) | 6.67 | 100 * | 30 |
LTRA (%) | 0 | 18 * | 90 |
ICS/LABA (%) | 0 | 90.91 | 100 |
OCS (%) | 0 | 0 | 10 |
TCS (%) | 6.67 | 100 * | 30 |
SABA (%) | 0 | 9.09 * | 100 |
T (%) | 0 | 0 | 10 |
D. pteronyssinus (%) | 0 | 90.91 | 80 |
D. farinae (%) | 0 | 90.91 | 80 |
L. destructor (%) | 0 | 54.55 | 50 |
B. tropicalis (%) | 0 | 81.82 | 40 |
A. siro (%) | 0 | 36.36 | 10 |
T. putrescentiae (%) | 0 | 45.45 | 50 |
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Escolar-Peña, A.; Delgado-Dolset, M.I.; Pablo-Torres, C.; Tarin, C.; Mera-Berriatua, L.; Cuesta Apausa, M.d.P.; González Cuervo, H.; Sharma, R.; Kho, A.T.; Tantisira, K.G.; et al. Specific microRNA Profile Associated with Inflammation and Lipid Metabolism for Stratifying Allergic Asthma Severity. Int. J. Mol. Sci. 2024, 25, 9425. https://doi.org/10.3390/ijms25179425
Escolar-Peña A, Delgado-Dolset MI, Pablo-Torres C, Tarin C, Mera-Berriatua L, Cuesta Apausa MdP, González Cuervo H, Sharma R, Kho AT, Tantisira KG, et al. Specific microRNA Profile Associated with Inflammation and Lipid Metabolism for Stratifying Allergic Asthma Severity. International Journal of Molecular Sciences. 2024; 25(17):9425. https://doi.org/10.3390/ijms25179425
Chicago/Turabian StyleEscolar-Peña, Andrea, María Isabel Delgado-Dolset, Carmela Pablo-Torres, Carlos Tarin, Leticia Mera-Berriatua, María del Pilar Cuesta Apausa, Heleia González Cuervo, Rinku Sharma, Alvin T. Kho, Kelan G. Tantisira, and et al. 2024. "Specific microRNA Profile Associated with Inflammation and Lipid Metabolism for Stratifying Allergic Asthma Severity" International Journal of Molecular Sciences 25, no. 17: 9425. https://doi.org/10.3390/ijms25179425