Abstract
Fusarium head blight (FHB), caused by the Fusarium graminearum species complex, is a destructive disease in wheat worldwide. The lack of FHB-resistant germplasm is a barrier in wheat breeding for resistance to FHB. Thinopyrum elongatum is an important relative that has been successfully used for the genetic improvement of wheat. In this study, a translocation line, YNM158, with the YM158 genetic background carrying a fragment of diploid Th. elongatum 7EL chromosome created using 60Co-γ radiation, showed high resistance to FHB under both field and greenhouse conditions. Transcriptome analysis confirmed that the horizontal transfer gene, encoding glutathione S-transferase (GST), is an important contributor to FHB resistance in the pathogen infection stage, whereas the 7EL chromosome fragment carries other genes regulated by F. graminearum during the colonization stage. Introgression of the 7EL fragment affected the expression of wheat genes that were enriched in resistance pathways, including the phosphatidylinositol signaling system, protein processing in the endoplasmic reticulum, plant–pathogen interaction, and the mitogen-activated protein kinase (MAPK) signaling pathway at different stages after F. graminearium infection. This study provides a novel germplasm for wheat resistance to FHB and new insights into the molecular mechanisms of wheat resistance to FHB.
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MDPI and ACS Style
Dai, Y.; Fei, W.; Chen, S.; Shi, J.; Ma, H.; Li, H.; Li, J.; Wang, Y.; Gao, Y.; Zhu, J.;
et al. Using Transcriptomics to Determine the Mechanism for the Resistance to Fusarium Head Blight of a Wheat-Th. elongatum Translocation Line. Int. J. Mol. Sci. 2024, 25, 9452.
https://doi.org/10.3390/ijms25179452
AMA Style
Dai Y, Fei W, Chen S, Shi J, Ma H, Li H, Li J, Wang Y, Gao Y, Zhu J,
et al. Using Transcriptomics to Determine the Mechanism for the Resistance to Fusarium Head Blight of a Wheat-Th. elongatum Translocation Line. International Journal of Molecular Sciences. 2024; 25(17):9452.
https://doi.org/10.3390/ijms25179452
Chicago/Turabian Style
Dai, Yi, Wenlin Fei, Shiqiang Chen, Juntao Shi, Haigang Ma, Haifeng Li, Jinfeng Li, Yonggang Wang, Yujiao Gao, Jinghuan Zhu,
and et al. 2024. "Using Transcriptomics to Determine the Mechanism for the Resistance to Fusarium Head Blight of a Wheat-Th. elongatum Translocation Line" International Journal of Molecular Sciences 25, no. 17: 9452.
https://doi.org/10.3390/ijms25179452
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