Spatiotemporal Dysregulation of Neuron–Glia Related Genes and Pro-/Anti-Inflammatory miRNAs in the 5xFAD Mouse Model of Alzheimer’s Disease
Abstract
:1. Introduction
2. Results
2.1. Age-Dependent Gene and miRNA Expression Variations Predominate over Genotype and Brain Region Differences in 5xFAD Mice
2.2. The 5xFAD Mice Overexpress Genes Whose Dysregulation Depends on Age and Region
2.3. miRNA Gene Ontology Indicates Common Pathways and Dysregulated Expression Levels in 5xFAD Mice with HPC and PFC Differences in 6- and 9-Month-Old Animals, but No Effective Clusters
2.4. Correlations between miRNAs and Genes Implicated in Neuroinflammation and Disease Onset/Progression Are Genotype-, Region-, and Age-Dependent
3. Discussion
Limitations
4. Materials and Methods
4.1. Animals
4.2. Mouse Brain Isolation and Dissection
4.3. RNA Extraction
4.4. Reverse Transcription and RT-qPCR for miRNAs
4.5. Reverse Transcription and RT-qPCR for Genes
4.6. Bioinformatic Analyses
4.7. miRNA–Targeting Network
4.8. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ianni, M.; Corraliza-Gomez, M.; Costa-Coelho, T.; Ferreira-Manso, M.; Inteiro-Oliveira, S.; Alemãn-Serrano, N.; Sebastião, A.M.; Garcia, G.; Diógenes, M.J.; Brites, D. Spatiotemporal Dysregulation of Neuron–Glia Related Genes and Pro-/Anti-Inflammatory miRNAs in the 5xFAD Mouse Model of Alzheimer’s Disease. Int. J. Mol. Sci. 2024, 25, 9475. https://doi.org/10.3390/ijms25179475
Ianni M, Corraliza-Gomez M, Costa-Coelho T, Ferreira-Manso M, Inteiro-Oliveira S, Alemãn-Serrano N, Sebastião AM, Garcia G, Diógenes MJ, Brites D. Spatiotemporal Dysregulation of Neuron–Glia Related Genes and Pro-/Anti-Inflammatory miRNAs in the 5xFAD Mouse Model of Alzheimer’s Disease. International Journal of Molecular Sciences. 2024; 25(17):9475. https://doi.org/10.3390/ijms25179475
Chicago/Turabian StyleIanni, Marta, Miriam Corraliza-Gomez, Tiago Costa-Coelho, Mafalda Ferreira-Manso, Sara Inteiro-Oliveira, Nuno Alemãn-Serrano, Ana M. Sebastião, Gonçalo Garcia, Maria José Diógenes, and Dora Brites. 2024. "Spatiotemporal Dysregulation of Neuron–Glia Related Genes and Pro-/Anti-Inflammatory miRNAs in the 5xFAD Mouse Model of Alzheimer’s Disease" International Journal of Molecular Sciences 25, no. 17: 9475. https://doi.org/10.3390/ijms25179475