The Neuroprotective Effect of Erythropoietin on the Optic Nerve and Spinal Cord in Rats with Experimental Autoimmune Encephalomyelitis through the Activation of the Extracellular Signal-Regulated Kinase 1/2 Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Histology
Hematoxylin and Eosin/Luxol Fast Blue
2.2. Immunohistochemistry
ERK Protein Expression
3. Discussion
4. Materials and Methods
4.1. EAE Model
4.2. Evaluation of the Evolution of EAE
4.3. Animals and Experimental Design
4.4. Obtain Optic Nerve and Spinal Cord
4.5. Automated Immunohistochemistry
4.6. Luxol Fast Blue Staining
4.7. Quantification of Histological Parameters
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pérez-Carranza, G.A.; Godínez-Rubí, J.M.; Márquez-Rosales, M.G.; Flores-Soto, M.E.; Bitzer-Quintero, O.K.; Ramírez-Anguiano, A.C.; Ramírez-Jirano, L.J. The Neuroprotective Effect of Erythropoietin on the Optic Nerve and Spinal Cord in Rats with Experimental Autoimmune Encephalomyelitis through the Activation of the Extracellular Signal-Regulated Kinase 1/2 Signaling Pathway. Int. J. Mol. Sci. 2024, 25, 9476. https://doi.org/10.3390/ijms25179476
Pérez-Carranza GA, Godínez-Rubí JM, Márquez-Rosales MG, Flores-Soto ME, Bitzer-Quintero OK, Ramírez-Anguiano AC, Ramírez-Jirano LJ. The Neuroprotective Effect of Erythropoietin on the Optic Nerve and Spinal Cord in Rats with Experimental Autoimmune Encephalomyelitis through the Activation of the Extracellular Signal-Regulated Kinase 1/2 Signaling Pathway. International Journal of Molecular Sciences. 2024; 25(17):9476. https://doi.org/10.3390/ijms25179476
Chicago/Turabian StylePérez-Carranza, Gloria Aleida, Juliana Marisol Godínez-Rubí, María Guadalupe Márquez-Rosales, Mario Eduardo Flores-Soto, Oscar Kurt Bitzer-Quintero, Ana Cristina Ramírez-Anguiano, and Luis Javier Ramírez-Jirano. 2024. "The Neuroprotective Effect of Erythropoietin on the Optic Nerve and Spinal Cord in Rats with Experimental Autoimmune Encephalomyelitis through the Activation of the Extracellular Signal-Regulated Kinase 1/2 Signaling Pathway" International Journal of Molecular Sciences 25, no. 17: 9476. https://doi.org/10.3390/ijms25179476