Raman Spectroscopy of Optically Trapped Living Human T Cell Subsets and Monocytes
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Cell Preparation
4.2. Raman Spectroscopy
4.3. Data Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
Cell Type | Diameter (Mean ± SD, µm) |
---|---|
CD4 | 6.6 ± 0.5 |
CD4stim | 10.9 ± 1.4 |
CD8 | 6.8 ± 0.6 |
CD8stim | 11.3 ± 2.2 |
Mono | 8.5 ± 0.4 |
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Compared Cell Types | Accuracy (Mean ± SD) | Approx. Discriminating Bands 1 (cm−1) |
---|---|---|
CD4:CD8 | 0.63 ± 0.48 | 1167, 1546, 1445 |
CD4:CD4stim | 0.92 ± 0.28 | 1163, 1546, 1389 |
CD8:CD8stim | 0.91 ± 0.29 | 1163, 1546, 628 |
CD4stim:CD8stim | 0.7 ± 0.46 | 1385, 623, 1457 |
CD4:Monocytes | 0.96 ± 0.19 | 1167, 1546, 1385 |
CD8:Monocytes | 0.96 ± 0.19 | 1167, 1546, 1389 |
CD4stim:Monocytes | 0.83 ± 0.37 | 1385, 1464, 623 |
CD8stim:Monocytes | 0.82 ± 0.38 | 1385, 623, 1154 |
Approx. Discriminating Bands (cm−1) | Tentative Assignation (Examples) [18] |
---|---|
1167 | C=N stretching in a quinoid ring, C-H in the plane, Tyrosine, Lipids |
1546 | NADH, C6-H deformation mode, Tryptophan, Amide II |
1445 | δ (CH2), δ (CH3), phospholipids |
1163 | Tyrosine, quinoid ring |
1389 | CH rocking, C-N stretching |
628 | Glycerol, Methionine |
1385 | CH3 band, CH rocking |
623 | Proteins |
1457 | Deoxyribose, Nucleic acids, proteins |
Cell Type | Number of Donors | Number of Measured Cells |
---|---|---|
CD4 | 16 | 1637 |
CD8 | 15 | 1687 |
CD4stim | 9 | 961 |
CD8stim | 8 | 856 |
Monocytes | 14 | 1453 |
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Nötzel, M.; Mahamid, M.; Kronstein-Wiedemann, R.; Ziemssen, T.; Akgün, K. Raman Spectroscopy of Optically Trapped Living Human T Cell Subsets and Monocytes. Int. J. Mol. Sci. 2024, 25, 9557. https://doi.org/10.3390/ijms25179557
Nötzel M, Mahamid M, Kronstein-Wiedemann R, Ziemssen T, Akgün K. Raman Spectroscopy of Optically Trapped Living Human T Cell Subsets and Monocytes. International Journal of Molecular Sciences. 2024; 25(17):9557. https://doi.org/10.3390/ijms25179557
Chicago/Turabian StyleNötzel, Martin, Maria Mahamid, Romy Kronstein-Wiedemann, Tjalf Ziemssen, and Katja Akgün. 2024. "Raman Spectroscopy of Optically Trapped Living Human T Cell Subsets and Monocytes" International Journal of Molecular Sciences 25, no. 17: 9557. https://doi.org/10.3390/ijms25179557