Boehmeria Nivea Extract (BNE-RRC) Reverses Epithelial-Mesenchymal Transition and Inhibits Anchorage-Independent Growth in Tumor Cells
Abstract
:1. Introduction
2. Results
2.1. The Effects of BNE-RRC on Cancer Cell Proliferation
2.2. The Effects of BNE-RRC Treatment on Neoplastic Cell Migration and Invasion
2.3. BNE-RRC Reverses Epithelial–Mesenchymal Transition in Oral Cancer Cells
2.4. BNE-RRC Induces and Maintains Epithelial Status in Cancer Cells by Inhibiting the Mesenchymal Phenotype
2.5. Induction of the Epithelial State by BNE-RRC Reverses EMT and Increases Sensitivity to Chemotherapy in Cancer Cells
2.6. Exploring the Effects of BNE-RRC Treatment on Telomerase Activity in Cancer Cells
2.7. Identification of Compounds through HPLC Analysis
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Soft Agar Assay
4.3. Western Blot Analysis
4.4. Wound-Healing Assay
4.5. Cell Migration Assay
4.6. Telomerase Activity Assay
4.7. HPLC Conditions
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, S.-L.; Hu, S.-W.; Lin, Y.-Y.; Liao, W.-L.; Yang, J.-J. Boehmeria Nivea Extract (BNE-RRC) Reverses Epithelial-Mesenchymal Transition and Inhibits Anchorage-Independent Growth in Tumor Cells. Int. J. Mol. Sci. 2024, 25, 9572. https://doi.org/10.3390/ijms25179572
Chen S-L, Hu S-W, Lin Y-Y, Liao W-L, Yang J-J. Boehmeria Nivea Extract (BNE-RRC) Reverses Epithelial-Mesenchymal Transition and Inhibits Anchorage-Independent Growth in Tumor Cells. International Journal of Molecular Sciences. 2024; 25(17):9572. https://doi.org/10.3390/ijms25179572
Chicago/Turabian StyleChen, Shiow-Ling, Suh-Woan Hu, Yuh-Yih Lin, Wen-Li Liao, and Jaw-Ji Yang. 2024. "Boehmeria Nivea Extract (BNE-RRC) Reverses Epithelial-Mesenchymal Transition and Inhibits Anchorage-Independent Growth in Tumor Cells" International Journal of Molecular Sciences 25, no. 17: 9572. https://doi.org/10.3390/ijms25179572