A Transcriptomic Analysis of Laryngeal Dysplasia
Abstract
:1. Introduction
2. Results
2.1. Dysregulated Genes Involving the Chemokine Signaling
2.2. Major Histocompatibility Complex Dysregulated Genes
2.3. Dysregulated Genes of B Cell Markers
2.4. Dysregulation of Lymphocyte Infiltrate Genes
2.5. Dysregulated Neutrophil Genes
2.6. Dysregulated Helper T Cell Genes
2.7. Dysregulated Genes Linked to Checkpoint Inhibitors
2.8. Dysregulated Tumor Genes
3. Discussion
4. Materials and Methods
4.1. Histopathological Analysis
4.2. Genetic Analysis
4.3. Statistical Analysis
4.4. Gene Expression Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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NCBI-Accession CODE | Gene Function | PDy | NPDy | Gene |
---|---|---|---|---|
NM_004591 | Chemokine signaling | UR | CCL20 | |
NM_005508 | Chemokine signaling | OE | CCR4 | |
NM_000435 | Tumor marker | UR | NOTCH3 | |
NM_003068 | Tumor marker steamness | UR | SNAI2 | |
NM_002122 | Antigen processing | OE | HLA-DQA1 | |
NR_026972 | Antigen-processing | OE | HLA-F-AS1 | |
NM_001192 | B cell marker | OE | TNFRSF17 | |
NM_144646 | B cell marker | OE | JCHAIN | |
NM_001783 | B cell-receptor signaling | OE | CD79A | |
NM_025239 | Checkpoint pathway | UR | PDCD1LG2 | |
NM_003151 | Helper T cell | UR | STAT4 | |
NM_005060 | Helper T cell | UR | RORC | |
NM_000717 | Neutrophil | OE | CA4 | |
NM_001780 | Lymphocyte infiltrate | OE | CD63 | |
NM_002727 | Lymphocyte infiltrate | OE | SRGN | |
NM_004106 | Lymphocyte infiltrate | OE | FCER1G |
p-Value 1 | PD | NPD | Characteristics |
---|---|---|---|
n = 15 (100%) | n = 31 (100%) | ||
0.2 | Sex | ||
1 (6.7%) | 8 (26%) | Female | |
14 (93%) | 23 (74%) | Male | |
0.2 | 66 (56, 70) | 58 (54, 64) | Age, median (IQR) |
0.8 | Smoking | ||
3 (20%) | 4 (13%) | Never | |
6 (40%) | 13 (42%) | Current | |
6 (40%) | 14 (45%) | Former | |
0.6 | Cancer site | ||
13 (87%) | 27 (87%) | Glottis | |
0 (0%) | 2 (6.5%) | Supraglottis | |
2 (13%) | 2 (6.5%) | Glottis + Supraglottis | |
0.2 | Side | ||
11 (73%) | 28 (90%) | Monolateral | |
4 (27%) | 3 (9.7%) | Bilateral | |
0.029 | Histological grade | ||
3 (20%) | 11 (35%) | SIN 1 | |
1 (6.7%) | 10 (32%) | SIN 2 | |
11 (73%) | 10 (32%) | SIN 3 | |
0.4 | Multifocal | ||
11 (73%) | 26 (84%) | No | |
4 (27%) | 5 (16%) | Yes |
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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Maffini, F.; Lepanto, D.; Chu, F.; Tagliabue, M.; Vacirca, D.; De Berardinis, R.; Gandini, S.; Vignati, S.; Ranghiero, A.; Taormina, S.; et al. A Transcriptomic Analysis of Laryngeal Dysplasia. Int. J. Mol. Sci. 2024, 25, 9685. https://doi.org/10.3390/ijms25179685
Maffini F, Lepanto D, Chu F, Tagliabue M, Vacirca D, De Berardinis R, Gandini S, Vignati S, Ranghiero A, Taormina S, et al. A Transcriptomic Analysis of Laryngeal Dysplasia. International Journal of Molecular Sciences. 2024; 25(17):9685. https://doi.org/10.3390/ijms25179685
Chicago/Turabian StyleMaffini, Fausto, Daniela Lepanto, Francesco Chu, Marta Tagliabue, Davide Vacirca, Rita De Berardinis, Sara Gandini, Silvano Vignati, Alberto Ranghiero, Sergio Taormina, and et al. 2024. "A Transcriptomic Analysis of Laryngeal Dysplasia" International Journal of Molecular Sciences 25, no. 17: 9685. https://doi.org/10.3390/ijms25179685