Comparison between USPIOs and SPIOs for Multimodal Imaging of Extracellular Vesicles Extracted from Adipose Tissue-Derived Adult Stem Cells
Abstract
:1. Introduction
2. Results
2.1. Nanoparticle Characterization
2.2. Toxicity and Internalization of NPs in ADAS
2.3. Imaging with Magnetic Resonance
2.4. Extraction of Extracellular Vesicles from ADAS-Labeled Cells
3. Discussion
4. Materials and Methods
4.1. Physical–Chemical Characterization of Nanoparticles
4.2. Internalization of Nanoparticles into Stem Cells
4.3. Magnetic Resonance Imaging
4.4. Extraction of Extracellular Vesicles from ADAS-Labeled Cells
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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USPIO in Distilled Water | USPIO in Agar Gel 2% | Resovist® in Distilled Water | Resovist® in Agar Gel 2% | |
---|---|---|---|---|
Size on Light Scattering (Size Peak nm ± SDev) | 9.5 ± 2.01 | 69.18 ± 1.56 | ||
Zeta Potential (mV ± ZDev) | −23.6 ± 1.7 | −31.8 ± 7.12 | ||
Size on Transmission Electron Microscopy (nm ± SDev) | 5.0± 0.01 | 70 ± 0.2 | ||
Transverse relaxivity (mM−1 s−1) | Not measured * | 103.3 ± 2.122 | 176.6 ± 1.868 | 274.9 ± 4.108 |
Longitudinal relaxivity (mM−1 s−1) | Not measured * | 0.4174 ± 0.018 | 1.736 ± 0.042 | 1.072 ± 0.07 |
Ratio r2/r1 | 247.484 | 101.73 | 256.436 |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Capuzzo, A.M.; Piccolantonio, G.; Negri, A.; Bontempi, P.; Lacavalla, M.A.; Malatesta, M.; Scambi, I.; Mariotti, R.; Lüdtke-Buzug, K.; Corsi, M.; et al. Comparison between USPIOs and SPIOs for Multimodal Imaging of Extracellular Vesicles Extracted from Adipose Tissue-Derived Adult Stem Cells. Int. J. Mol. Sci. 2024, 25, 9701. https://doi.org/10.3390/ijms25179701
Capuzzo AM, Piccolantonio G, Negri A, Bontempi P, Lacavalla MA, Malatesta M, Scambi I, Mariotti R, Lüdtke-Buzug K, Corsi M, et al. Comparison between USPIOs and SPIOs for Multimodal Imaging of Extracellular Vesicles Extracted from Adipose Tissue-Derived Adult Stem Cells. International Journal of Molecular Sciences. 2024; 25(17):9701. https://doi.org/10.3390/ijms25179701
Chicago/Turabian StyleCapuzzo, Arnaud M., Giusi Piccolantonio, Alessandro Negri, Pietro Bontempi, Maria A. Lacavalla, Manuela Malatesta, Ilaria Scambi, Raffaella Mariotti, Kerstin Lüdtke-Buzug, Mauro Corsi, and et al. 2024. "Comparison between USPIOs and SPIOs for Multimodal Imaging of Extracellular Vesicles Extracted from Adipose Tissue-Derived Adult Stem Cells" International Journal of Molecular Sciences 25, no. 17: 9701. https://doi.org/10.3390/ijms25179701